BBa_J70368BBa_J70368 Version 1 (Component)I13502 or K093005 (B0034+E1010) rfp without PsrI sites
Holin, T4BBa_K112003 Version 1 (Component)T4 holin, without start or stop codon, berkeley standard
BBa_J329004BBa_J329004 Version 1 (Component)Quorum Sensing Sender Part for Use in BBa_J329003
BBa_K415004BBa_K415004 Version 1 (Component)P_const(high) : RBS : LuxR : Term : pLux/CI-OR
BBa_M31748BBa_M31748 Version 1 (Component)Gene VII w/o Genes IX RBS or VIII Promoter
BBa_J70552BBa_J70552 Version 1 (Component)J70551.J70549 (or J70548.J70550.J70549) composite part
BBa_K750108BBa_K750108 Version 1 (Component)PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose
BBa_K2123301BBa_K2123301 Version 1 (Component)Novel Synthetic Phytochelatin codon optimized for E. coli expression
KanR-BA-BfBBa_K349012 Version 1 (Component)KanR-BA-BfuAI (for construction of BA BioBytes 2.0)
BBa_K199070BBa_K199070 Version 1 (Component)I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC
BBa_M31002BBa_M31002 Version 1 (Component)Refactored Gene 2 without Gene 10's Promoter or RBS
BBa_K415003BBa_K415003 Version 1 (Component)P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR
BBa_K112222BBa_K112222 Version 1 (Component){< ihfB >} The ihf beta gene without start or stop codons, BBb format
BBa_K563010BBa_K563010 Version 1 (Component)Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway
BBa_J70556BBa_J70556 Version 1 (Component)BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554
BBa_K1341011BBa_K1341011 Version 1 (Component)OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)
BBa_K812130BBa_K812130 Version 1 (Component)Citrine reporter with a Kozak sequence for expression in Xenopus
BBa_K812120BBa_K812120 Version 1 (Component)IaaH on PSC2+ Ready for use in xenopus tropicalis egg
BBa_K648125BBa_K648125 Version 1 (Component)Or tester with cI repressor with slightly weak RBS
BBa_K648126BBa_K648126 Version 1 (Component)Or tester with cI repressor with very weak RBS
BBa_K251000BBa_K251000 Version 1 (Component)coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)
BBa_I714001BBa_I714001 Version 1 (Component)trwC of R388 ( relaxase or nickase of the R388 conjugative plasmid )
BBa_K415002BBa_K415002 Version 1 (Component)P_const(high) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
xis domainBBa_K112203 Version 1 (Component){< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21
BBa_J70559BBa_J70559 Version 1 (Component)N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040
BBa_K363002BBa_K363002 Version 1 (Component)A calcium dependent response element binding site for the Crz1 activator
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K199071BBa_K199071 Version 1 (Component)I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC
BBa_K802003BBa_K802003 Version 1 (Component)Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>
BBa_K2016006BBa_K2016006 Version 1 (Component)Superfolder GFP under control or medium constitutive promoter and RyhB siRNA domain
BBa_K415001BBa_K415001 Version 1 (Component)P_const(low/med) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term
BBa_K750115BBa_K750115 Version 1 (Component)pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)
BBa_I718001BBa_I718001 Version 1 (Component)ech My new test generator part Feruloyl CoA hydratase for vanilin
BBa_K648130BBa_K648130 Version 1 (Component)Pr of Or tester with cI repressor with very weak RBS
BBa_K648129BBa_K648129 Version 1 (Component)Pr of Or tester with cI repressor with slightly weak RBS
BBa_K750112BBa_K750112 Version 1 (Component)GFP(lva) expression system controlled by cI or atc(RBS strength:1.0)
BBa_K2020051BBa_K2020051 Version 1 (Component)wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G
BBa_K415008BBa_K415008 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(lac) IQ : luxR : Term : pLux
BBa_K415009BBa_K415009 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : P(tetR) : RBS-luxR : Term : pLux
BBa_K2123115BBa_K2123115 Version 1 (Component)Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014
BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
BBa_I20292BBa_I20292 Version 1 (Component)There is no limit to what a man can do or where he can go if...
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
BBa_J85010BBa_J85010 Version 1 (Component)PLux/cI-OR Promoter
Adapter BiBBa_K1807015 Version 1 (Component)This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.