BBa_J04676
BBa_J04676 Version 1 (Component)
R0010 with Anti-Sense Theophillline Induced Antiswitch ("Off Switch")

BBa_I3600
BBa_I3600 Version 1 (Component)
Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)

BBa_K1085020
BBa_K1085020 Version 1 (Component)
RBS Start-codon EstA SpiderSilkSubunitN1 SpiderSilkSubunitN2 SilkTail Strep-tag Stop-codon

BBa_K1085019
BBa_K1085019 Version 1 (Component)
RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2 SilkTail Stop-codon

BBa_K1085028
BBa_K1085028 Version 1 (Component)
RBS Start-codon SpiderSilkSubunitE1 SpiderSilkSubunitE2 x2 SilkTail Strep-tag Stop-codon

BBa_I13038
BBa_I13038 Version 1 (Component)
Lux I/R Sender and Receiver w/ TetR regulation

BBa_J70659
BBa_J70659 Version 1 (Component)
N-terminal 6 strep fusion test for GFP J70610w n-terminalstreptag inserts

BBa_I13037
BBa_I13037 Version 1 (Component)
Lux I/R Sender and Receiver w/ TetR regulation

BBa_K1090000
BBa_K1090000 Version 1 (Component)
AHL signal sender with RFP reporter under lac promoter control

BBa_K2033002
BBa_K2033002 Version 1 (Component)
isovaleryl-HSL, 3-methyl-N-[(3S)-2-oxooxolan-3-yl]butanamide Sender- BjaI

BBa_K359005
BBa_K359005 Version 1 (Component)
Senses AIP and has RFP reporter (from Agr quorum sensing system)

BBa_J70652
BBa_J70652 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_I3521
BBa_I3521 Version 1 (Component)
Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)

BBa_K582000
BBa_K582000 Version 1 (Component)
500bp of Spacer/Repeat Region of the CRISPR genome present in Strep. thermophilus LMD9 strain

BBa_J70653
BBa_J70653 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K658014
BBa_K658014 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-5 Driven by PLlac 0-1

BBa_K1159309
BBa_K1159309 Version 1 (Component)
19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]

BBa_K658013
BBa_K658013 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-3 Driven by PLlac 0-1

BBa_K2033004
BBa_K2033004 Version 1 (Component)
(3S)-3-[(2-oxo-3-phenylpropyl)amino]oxolan-2-one (3-phenyl-HSL) Sender- BraI

Strep-Tag
BBa_I757014 Version 1 (Component)
Strep-Tag II affinity tag

BBa_I1060
BBa_I1060 Version 1 (Component)
Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)

BBa_K658015
BBa_K658015 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-3/5 Driven by PLlac 0-1

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_J47421
BBa_J47421 Version 1 (Component)
constitutive luxI sender device

BBa_K091135
BBa_K091135 Version 1 (Component)
Las Sender Testing Device

BBa_I731017
BBa_I731017 Version 1 (Component)
Ptet bicistronic 3OC6HSL Sender + mCherry

BBa_I9023
BBa_I9023 Version 1 (Component)
sender (luxI) B0034.C0061.B0015

BBa_I9027
BBa_I9027 Version 1 (Component)
sender (rhlI) B0034.C0070.B0015

BBa_F1760
BBa_F1760 Version 1 (Component)
3OC₁₄HSL Sender Device

BBa_I719021
BBa_I719021 Version 1 (Component)
Constitutive 3OC₆HSL Sender Device

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.