BBa_K654061
BBa_K654061 Version 1 (Component)
Wax Ester Synthase with 8-His Tag

BBa_K542010
BBa_K542010 Version 1 (Component)
Enhanced Lumazine Synthase (ELS)

BBa_K654067
BBa_K654067 Version 1 (Component)
Wax Ester Synthase + Terminator

BBa_K654073
BBa_K654073 Version 1 (Component)
psbA2 controlled Wax Ester Synthase

PenI inv
BBa_K566010 Version 1 (Component)
PenI repressor optimized for E. coli (inverted sequence)

BBa_K764022
BBa_K764022 Version 1 (Component)
Hyaluronan synthase

BBa_K764025
BBa_K764025 Version 1 (Component)
Hyaluronan synthase + BBa_B0014

BBa_K764029
BBa_K764029 Version 1 (Component)
BBa_J13002_Hyaluronan synthase_BBa_B0014

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_M11060
BBa_M11060 Version 1 (Component)
pfaD omega-3 polyunsaturated fatty acid synthase

BBa_M11061
BBa_M11061 Version 1 (Component)
pfaD omega-3 polyunsaturated fatty acid synthase (mutated)

BBa_M11062
BBa_M11062 Version 1 (Component)
pfaC gene for omega-3 polyunsaturated fatty acid synthase

BBa_M11063
BBa_M11063 Version 1 (Component)
pfaE omega-3 polyunsaturated fatty acid synthase (mutated)

BBa_M11053
BBa_M11053 Version 1 (Component)
linalool synthase for floral smell biosynthesis (LIS)

BBa_M11080
BBa_M11080 Version 1 (Component)
silE (coding sequence for Silver binding protein)

BBa_M11081
BBa_M11081 Version 1 (Component)
cutA (coding sequence for copper binding protein)

BBa_M11083
BBa_M11083 Version 1 (Component)
MerP, coding sequence for mercuric ion binding protein

BBa_M12066
BBa_M12066 Version 1 (Component)
Cellulose Synthase Gene

BBa_C100012
BBa_C100012 Version 1 (Component)
ORF of the myrcene synthase odorant enzyme

BBa_P100112
BBa_P100112 Version 1 (Component)
Myrcene Synthase ORF with RBS

BBa_P101112
BBa_P101112 Version 1 (Component)
Myrcene Synthase RBS.ORF.Term

BBa_P111112
BBa_P111112 Version 1 (Component)
Myrcene Synthase Expression Cassette (R0040)

BBa_P121112
BBa_P121112 Version 1 (Component)
Myrcene Synthase Expression Cassette (F2620)

BBa_K294000
BBa_K294000 Version 1 (Component)
This is the coding sequence for the heat shock protein hsp15 from E. coli

BBa_K294125
BBa_K294125 Version 1 (Component)
A coding sequence for one of the heat shock protein:hchA

BBa_P100212
BBa_P100212 Version 1 (Component)
Myrcene Synthase ORF and RBS (B0029)

BBa_C100017
BBa_C100017 Version 1 (Component)
Myrcene Synthase (B0034 optimized)

BBa_P100117
BBa_P100117 Version 1 (Component)
Myrcene Synthase ORF with RBS (optimized)

BBa_P101117
BBa_P101117 Version 1 (Component)
Myrcene Synthase ORF with RBS and Term (optimized)

BBa_P111117
BBa_P111117 Version 1 (Component)
Myrcene Synthase complete construct (R0040, B0034 optimized)

BBa_P121117
BBa_P121117 Version 1 (Component)
Myrcene Synthase complete construct (F2620, B0034 optimized)

BBa_K249002
BBa_K249002 Version 1 (Component)
Lumazine Synthase

BBa_K249003
BBa_K249003 Version 1 (Component)
Lumazine Synthase

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

BBa_K231001
BBa_K231001 Version 1 (Component)
Phytochelatin Synthase, HA-tag

BBa_K252007
BBa_K252007 Version 1 (Component)
OmpA target sequence for MicA

BBa_K252008
BBa_K252008 Version 1 (Component)
OmpC target sequence for MicC

BBa_K252009
BBa_K252009 Version 1 (Component)
OmpF target sequence for MicF

BBa_K252010
BBa_K252010 Version 1 (Component)
dppA target sequence for GcvB

BBa_K252011
BBa_K252011 Version 1 (Component)
oppA target sequence for GcvB

BBa_K252012
BBa_K252012 Version 1 (Component)
GalK target sequence for Spot42

BBa_K252013
BBa_K252013 Version 1 (Component)
ftsZ target sequence for DicF

BBa_K252014
BBa_K252014 Version 1 (Component)
ptsG target sequence for sgrS

BBa_K331000
BBa_K331000 Version 1 (Component)
IPTG inducible Lumazine Synthase

BBa_K331018
BBa_K331018 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with N-Terminal Arginine Tag)

BBa_K331019
BBa_K331019 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with C-Terminal Arginine Tag)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.