BBa_K2144010
BBa_K2144010 Version 1 (Component)
Coding sequence for Esp regulated by T7-promoter

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K2144012
BBa_K2144012 Version 1 (Component)
Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter

BBa_K2023009
BBa_K2023009 Version 1 (Component)
Gaussia luciferase coding sequence optimized for use in <i>E.coli</i> DH5-Alpha

BBa_K2082251
BBa_K2082251 Version 1 (Component)
Sequence for rpoZ Knock-Out in E. coli through CRISPR/Cas9

BBa_K1985010
BBa_K1985010 Version 1 (Component)
Sequence coding for amyloid Sup35 residues 1-61

BBa_K1985011
BBa_K1985011 Version 1 (Component)
Sequence coding for amyloid Sup35 residues 1-61 and Cytochrome b562

BBa_K1985013
BBa_K1985013 Version 1 (Component)
Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter

BBa_K1985014
BBa_K1985014 Version 1 (Component)
Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

Cripto-1
BBa_K1908001 Version 1 (Component)
Coding sequence for Homo sapien Cripto-1 membrane protein.

BBa_J331010
BBa_J331010 Version 1 (Component)
Linking Sequence for Metallothionein (CRS5) and Glutathione S-Transferase (GST)

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Collection)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_M34569
BBa_M34569 Version 1 (Component)
Coding Sequence for Phytochelatin Synthase found in Arabidopsis thaliana.

BBa_M34570
BBa_M34570 Version 1 (Component)
Coding Sequence for ARR1/ACR1 from S. Cerevisiae

BBa_M34571
BBa_M34571 Version 1 (Component)
Coding Sequence for ARR2/ACR2 from S. Cerevisiae.

BBa_M34572
BBa_M34572 Version 1 (Component)
Coding Sequence for Arsenic Transport 1 found in Arabadopsis Thaliana

BBa_M34573
BBa_M34573 Version 1 (Component)
Coding Sequence for Arsenic Transport 2, from Arabadopsis Thaliana

BBa_K541516
BBa_K541516 Version 1 (Component)
Reflectin1A for B.subtiis with SacB signal sequence

BBa_K541536
BBa_K541536 Version 1 (Component)
Reflectin 1A for E.coli with TAT signal sequence (Constitutive)

MTS
BBa_K678025 Version 1 (Component)
Mitochondrial targeting sequence for Aspergillus nidulans

PenI inv
BBa_K566010 Version 1 (Component)
PenI repressor optimized for E. coli (inverted sequence)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_M11080
BBa_M11080 Version 1 (Component)
silE (coding sequence for Silver binding protein)

BBa_M11081
BBa_M11081 Version 1 (Component)
cutA (coding sequence for copper binding protein)

BBa_M11083
BBa_M11083 Version 1 (Component)
MerP, coding sequence for mercuric ion binding protein

BBa_K294000
BBa_K294000 Version 1 (Component)
This is the coding sequence for the heat shock protein hsp15 from E. coli

BBa_K294125
BBa_K294125 Version 1 (Component)
A coding sequence for one of the heat shock protein:hchA

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

BBa_K252007
BBa_K252007 Version 1 (Component)
OmpA target sequence for MicA

BBa_K252008
BBa_K252008 Version 1 (Component)
OmpC target sequence for MicC

BBa_K252009
BBa_K252009 Version 1 (Component)
OmpF target sequence for MicF

BBa_K252010
BBa_K252010 Version 1 (Component)
dppA target sequence for GcvB

BBa_K252011
BBa_K252011 Version 1 (Component)
oppA target sequence for GcvB

BBa_K252012
BBa_K252012 Version 1 (Component)
GalK target sequence for Spot42

BBa_K252013
BBa_K252013 Version 1 (Component)
ftsZ target sequence for DicF

BBa_K252014
BBa_K252014 Version 1 (Component)
ptsG target sequence for sgrS

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.