BBa_I732020BBa_I732020 Version 1 (Component)lacZ alpha (fragment RBS+ TERM+)
BBa_K1332003BBa_K1332003 Version 1 (Component)The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon
BBa_K1332004BBa_K1332004 Version 1 (Component)The 5?? side of the intron(+exon fragment) from td gene of T4 phage
BBa_K1332005BBa_K1332005 Version 1 (Component)The 3' side of the intron(+exon fragment) from td gene of T4 phage
BBa_E0029BBa_E0029 Version 1 (Component)lacZalpha fragment
BBa_K323072BBa_K323072 Version 1 (Component)N-split fragment of mCitrine with stop
BBa_K323011BBa_K323011 Version 1 (Component)N-split fragment of mCerulean with stop
BBa_K323012BBa_K323012 Version 1 (Component)C-split fragment of mCerulean with ATG
BBa_K323032BBa_K323032 Version 1 (Component)C-split fragment of mCitrine with ATG and link
BBa_K323037BBa_K323037 Version 1 (Component)C-split fragment of mCerulean with ATG and link
BBa_K323055BBa_K323055 Version 1 (Component)C-split fragment of mCitrine with ATG
BBa_K1510119BBa_K1510119 Version 1 (Component)A truncated INPNC anchor protein that binds fragments on the cell surface
BBa_K1982003BBa_K1982003 Version 1 (Component)CIBN(the N-terminal fragment of CIB1)
tnaA-homolBBa_K1919400 Version 1 (Component)tnaA-homologous substitution fragment
BBa_K1976049BBa_K1976049 Version 1 (Component)Trypsin Fragment of Colicin E2
BBa_K1976051BBa_K1976051 Version 1 (Component)Trypsin Fragment of Colicin E2 with B0034 as a RBS
BBa_K1976055BBa_K1976055 Version 1 (Component)T7 generator of the trypsin fragment of the Colicin E2 DNase
BBa_K1945005BBa_K1945005 Version 1 (Component)C-terminal fragment of T7 RNA Polymerase
BBa_K1945014BBa_K1945014 Version 1 (Component)Reverse complement of N-terminal fragment of split T7 RNA polymerase
BBa_K1945004BBa_K1945004 Version 1 (Component)N-terminal fragment of split T7 RNA polymerase
BBa_K329005BBa_K329005 Version 1 (Component)Strong RBS (B0034) - LacZ-alpha fragment (I732006)
BBa_K329006BBa_K329006 Version 1 (Component)Weak RBS (B0031) - LacZ alpha fragment (I732006)
BBa_J70634BBa_J70634 Version 1 (Component)Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment
BBa_J70635BBa_J70635 Version 1 (Component)Downstream genomic DNA fragment of Mesoplasma florum for recombination experiment
BBa_K784015BBa_K784015 Version 1 (Component)Phage Lambda fragment 1 - head proteins
BBa_K784016BBa_K784016 Version 1 (Component)Phage Lambda fragment 2 - tail proteins
BBa_K784017BBa_K784017 Version 1 (Component)Phage Lambda fragment 3 - tail tip proteins
BBa_K784018BBa_K784018 Version 1 (Component)Phage Lambda fragment 4 - tail fiber proteins
BBa_K784019BBa_K784019 Version 1 (Component)Phage Lambda fragment 5 - recombination
BBa_K784020BBa_K784020 Version 1 (Component)Phage Lambda fragment 6 - regulation
BBa_K784021BBa_K784021 Version 1 (Component)Phage Lambda fragment 7 - replication and lytic regulation
BBa_K784022BBa_K784022 Version 1 (Component)Phage Lambda fragment 8 - lytic proteins
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K157003BBa_K157003 Version 1 (Component)Anti-NIP singlechain Fv-Fragment
BBa_K152008BBa_K152008 Version 1 (Component)250 bp fragment from the Zea mays gene TB1
BBa_K242050BBa_K242050 Version 1 (Component)cos-containing P4 DNA fragment