BBa_K530028
BBa_K530028 Version 1 (Component)
pRSBB403 HIS3 Integrating Yeast Shuttle Vector

BBa_K530029
BBa_K530029 Version 1 (Component)
pRSBB404 TRP1 Integrating Yeast Shuttle Vector

BBa_K530030
BBa_K530030 Version 1 (Component)
pRSBB405 LEU2 Integrating Yeast Shuttle Vector

BBa_K530031
BBa_K530031 Version 1 (Component)
pRSBB406 URA3 Integrating Yeast Shuttle Vector

BBa_K530032
BBa_K530032 Version 1 (Component)
pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector

BBa_K530033
BBa_K530033 Version 1 (Component)
pRSBB414 TRP1 CEN/ARS Yeast Shuttle Vector

BBa_K530034
BBa_K530034 Version 1 (Component)
pRSBB415 LEU2 CEN/ARS Yeast Shuttle Vector

BBa_K530035
BBa_K530035 Version 1 (Component)
pRSBB416 URA3 CEN/ARS Yeast Shuttle Vector

BBa_K530036
BBa_K530036 Version 1 (Component)
pRSBB423 HIS3 2Micron Episomal Yeast Shuttle Vector

BBa_K530037
BBa_K530037 Version 1 (Component)
pRSBB424 TRP1 2Micron Episomal Yeast Shuttle Vector

BBa_K530038
BBa_K530038 Version 1 (Component)
pRSBB425 LEU2 2Micron Episomal Yeast Shuttle Vector

BBa_K530039
BBa_K530039 Version 1 (Component)
pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector

BBa_K535007
BBa_K535007 Version 1 (Component)
pBBR1MCS-5 -> broad-host-range cloning vector

BBa_K896003
BBa_K896003 Version 1 (Component)
Sulfide quinone reductase(SQR) on neutral site 2 vector

BBa_K950010
BBa_K950010 Version 1 (Component)
pRS315 shuttle vector

BBa_K950011
BBa_K950011 Version 1 (Component)
pRS316 shuttle vector

BBa_J72202
BBa_J72202 Version 1 (Component)
ampicillin resistant p15A vector

BBa_J72203
BBa_J72203 Version 1 (Component)
kanamycin resistant split R6K vector

BBa_J72204
BBa_J72204 Version 1 (Component)
ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT

BBa_J72205
BBa_J72205 Version 1 (Component)
ampicillin resistant split ColE2 vector

BBa_J72206
BBa_J72206 Version 1 (Component)
ampicillin resistant split R6K vector

BBa_J72207
BBa_J72207 Version 1 (Component)
ampicillin resistant pUC vector

BBa_J72209
BBa_J72209 Version 1 (Component)
kanamycin + ampicillin resistant p15A vector for 2ab assembly

BBa_J72211
BBa_J72211 Version 1 (Component)
ampicillin resistant p15A vector with dapD

BBa_J72212
BBa_J72212 Version 1 (Component)
spectinomycin resistant pUC vector

BBa_J72218
BBa_J72218 Version 1 (Component)
kanamycin resistant p15A vector

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Eukaryotic
BBa_K157040 Version 1 (Component)
Eukaryotic terminator vector (very similar to pSB1AK8)

Transfecti
BBa_K157041 Version 1 (Component)
Transfection vector (similar to pSB1AK8)

BBa_K106342
BBa_K106342 Version 1 (Component)
AB shuttle vector

BBa_K106343
BBa_K106343 Version 1 (Component)
BD shuttle vector

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

BBa_K146000
BBa_K146000 Version 1 (Component)
pCR2.1 PCR product cloning vector

BBa_K106672
BBa_K106672 Version 1 (Component)
AarI AD acceptor vector (pRS305, Gal1P, Adh1t)

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_K106697
BBa_K106697 Version 1 (Component)
AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)

BBa_K106698
BBa_K106698 Version 1 (Component)
AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)

T7p
BBa_K133041 Version 1 (Component)
T7 promoter expression plasmid backbone

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

BBa_K106701
BBa_K106701 Version 1 (Component)
pCR2.1 TOPO Vector (to contain AarI inserts)

BBa_K165061
BBa_K165061 Version 1 (Component)
pRS304* yeast shuttle vector, TRP1 selection

BBa_K090402
BBa_K090402 Version 1 (Component)
B. subtilis Episomal Vector with Constitutive GFP

BBa_K165060
BBa_K165060 Version 1 (Component)
pRS303 yeast shuttle vector, HIS3

BBa_K165062
BBa_K165062 Version 1 (Component)
pRS305 yeast shuttle vector, LEU2 selection

BBa_K165063
BBa_K165063 Version 1 (Component)
pRS306 yeast shuttle vector, URA3 selection

BBa_K090403
BBa_K090403 Version 1 (Component)
Gram-positive Shuttle Vector for Chromosomal Integration

BBa_K112134
BBa_K112134 Version 1 (Component)
Cam-Amp assembly vector

BBa_K112143
BBa_K112143 Version 1 (Component)
Entry vector with OriV replication origin

AD default
BBa_K106400 Version 1 (Component)
Default insert for AarI AD acceptor vectors