BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K880004BBa_K880004 Version 1 (Component)LVA Tag in RFC25
7x HisBBa_T2001 Version 1 (Component)7x His tag head domain
c-MycBBa_T2006 Version 1 (Component)c-Myc tail domain (EQKLISEEDL)
BBa_K137064BBa_K137064 Version 1 (Component)Promoter with (TA)9 repeat in front of RBS-LacZ-TT
BBa_K137063BBa_K137063 Version 1 (Component)Promoter with (TA)10 repeat in front of RBS-LacZ-TT
BBa_K936022BBa_K936022 Version 1 (Component)Cutinase and polyhistidine tag
BBa_K809321BBa_K809321 Version 1 (Component)GAL promoter + YFP_SPtim21 + ADH1 terminator
BBa_K809308BBa_K809308 Version 1 (Component)GAL promoter + RFP_SPzim17 + ADH1 terminator
BBa_K1845001BBa_K1845001 Version 1 (Component)Miraculin (Yeast codon optimised + His-tag)
BBa_I724005BBa_I724005 Version 1 (Component)Elowitz repressilator with added degradation tag
BBa_K1685002BBa_K1685002 Version 1 (Component)aeBlue with LVA tag and double terminator
BBa_K2172009BBa_K2172009 Version 1 (Component)Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator
BBa_K1441013BBa_K1441013 Version 1 (Component)DNA ligase from Escherichia coli with His-tag INSERT
BBa_K542011BBa_K542011 Version 1 (Component)Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg)
BBa_K1974011BBa_K1974011 Version 1 (Component)T7 Promoter+RBS+Hv1a+linker+6X His-Tag
BBa_K1974013BBa_K1974013 Version 1 (Component)T7 Promoter+RBS+OAIP+linker+6X His-Tag
BBa_K809710BBa_K809710 Version 1 (Component)GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator
BBa_K1974022BBa_K1974022 Version 1 (Component)T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag
BBa_K1974021BBa_K1974021 Version 1 (Component)T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag
BBa_K1974023BBa_K1974023 Version 1 (Component)T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag
BBa_K1036003BBa_K1036003 Version 1 (Component)lux pL controlled luxR with lux pR controlled gfp (LVA-tag)
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
BBa_K2123112BBa_K2123112 Version 1 (Component)Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
BBa_K1974033BBa_K1974033 Version 1 (Component)T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag
BBa_K2123115BBa_K2123115 Version 1 (Component)Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014
BBa_K2144011BBa_K2144011 Version 1 (Component)Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter
BBa_K2066500BBa_K2066500 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.