BBa_K626009
BBa_K626009 Version 1 (Component)
DBD designed for RARE in CD38 - EcR LBD

BBa_I13607
BBa_I13607 Version 1 (Component)
Lac YFP Tet CFP (Ly+Tc+)

BBa_K1475010
BBa_K1475010 Version 1 (Component)
dxs+LIMS1+appY regulated by the tet promoter

BBa_J119124
BBa_J119124 Version 1 (Component)
trpR, tryptophan-repressor, represses the Promoter-tryp when tryp is high

BBa_I20530
BBa_I20530 Version 1 (Component)
Consensus promoter for phage K1-5 RNA polymerase

HerE
BBa_K1615045 Version 1 (Component)
Heroin Esterase in RFC25

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_I11031
BBa_I11031 Version 1 (Component)
excisionase from E. coli phage P22 (removes prophage from host genome)

BBa_K855003
BBa_K855003 Version 1 (Component)
coding sequence for pvdQ with histag added to the C-terminal followed by stop codon

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K1753000
BBa_K1753000 Version 1 (Component)
the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

BBa_K631001
BBa_K631001 Version 1 (Component)
ODR-7 (promoter; for use in C. elegans)

BBa_K1159102
BBa_K1159102 Version 1 (Component)
C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

BBa_K892007
BBa_K892007 Version 1 (Component)
red light sensing promoter driving the C1 inverter

BBa_K1758312
BBa_K1758312 Version 1 (Component)
Chromium responsive promoter with UTR+RBS and sfGFP

BBa_K094100
BBa_K094100 Version 1 (Component)
cheZ gene

BBa_K1051004
BBa_K1051004 Version 1 (Component)
Stop codon free mOrange in RFC[23] standard

p(osmY)
BBa_J45993 Version 1 (Component)
Minimal stationary phase osmY promoter

DnaJ/GrpE
BBa_K205003 Version 1 (Component)
DnaJ and GrpE are chaperone proteins that assists with polypeptide folding

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_E7103
BBa_E7103 Version 1 (Component)
Reporter Device Powered by the Virtual Machine (VM1.0)

BBa_K1159303
BBa_K1159303 Version 1 (Component)
Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]

BBa_K1969018
BBa_K1969018 Version 1 (Component)
The linker sequence between GAL1 promoter and ADRB2 (with His-tag)

BBa_K1969008
BBa_K1969008 Version 1 (Component)
The linker sequence between ADH1 promoter and Nano-lantern(cAMP-1.6)

BBa_K1116003
BBa_K1116003 Version 1 (Component)
Tre-LacI with miR-FF5 and miR-FF3 target

BBa_I13107
BBa_I13107 Version 1 (Component)
Low output control (lac QPI, Elowitz RBS)

eGFP+TmTHP
BBa_K193203 Version 1 (Component)
TmTHP with EGFP for fusion of Antifreeze protein

BBa_K1613017
BBa_K1613017 Version 1 (Component)
K1613017 is a new part which can detect lead (Pb) ions in the liquid.

BBa_K1699002
BBa_K1699002 Version 1 (Component)
gRNA for SaCas9 targeting human ubiquitin B gene

BBa_K1187000
BBa_K1187000 Version 1 (Component)
Human insulin gene, optimized for expression in Pichia pastoris

BBa_J58101
BBa_J58101 Version 1 (Component)
Promoter activated synergistically by cI and CRP with the reporter RFP

BBa_M30060
BBa_M30060 Version 1 (Component)
Refactoring M13K07

BBa_S04145
BBa_S04145 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022+I725023)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

pFig2c
BBa_K1829002 Version 1 (Component)
pFig2c: Mating factor alpha responsive promoter

BBa_I11020
BBa_I11020 Version 1 (Component)
integrase from E. coli phage lambda

BBa_M30059
BBa_M30059 Version 1 (Component)
Refactoring M13K07

BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

BBa_K1613009
BBa_K1613009 Version 1 (Component)
A composite part that can detect the recA.

BBa_K1613010
BBa_K1613010 Version 1 (Component)
A composite part that can detect the recA.

BBa_K1613011
BBa_K1613011 Version 1 (Component)
A composite part that can detect the recA.

BBa_K1613008
BBa_K1613008 Version 1 (Component)
A composite part that can detect the recA.

BBa_K740003
BBa_K740003 Version 1 (Component)
promoter with loxP sites that can switch the reporter

BBa_K112228
BBa_K112228 Version 1 (Component)
{pelB>} The pelB leader without stop codon, BBb format

BBa_K1758377
BBa_K1758377 Version 1 (Component)
Biosensor device for detection of GHB and GBL