BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

BBa_K769002
BBa_K769002 Version 1 (Component)
Kinase domain of EnvZ

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR

BBa_K116520
BBa_K116520 Version 1 (Component)
GFP under control of RpaA activated pOmpF promoter

BBa_K116523
BBa_K116523 Version 1 (Component)
GFP under control of RpaA activated pOmpC promoter

BBa_K116002
BBa_K116002 Version 1 (Component)
measurement of NhaA promoter activity

pR-RFP
BBa_K093010 Version 1 (Component)
lambda pR-RFP generator

BBa_K112021
BBa_K112021 Version 1 (Component)
Lambda phage lysis device - no promoter

BBa_K112022
BBa_K112022 Version 1 (Component)
Lambda phage lysis device - no promoter

BBa_K112023
BBa_K112023 Version 1 (Component)
Lambda phage lysis device - no promoter

BBa_K112125
BBa_K112125 Version 1 (Component)
OriV origin of replication

BBa_K112147
BBa_K112147 Version 1 (Component)
lambda lysis device controlled by magnesium promoter

BBa_K112146
BBa_K112146 Version 1 (Component)
lambda lysis device controlled by PmgtCB magnesium promoter

cro
BBa_K177009 Version 1 (Component)
lambda antirepressor cro

BBa_M11082
BBa_M11082 Version 1 (Component)
ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)

BBa_M11051
BBa_M11051 Version 1 (Component)
Captures light energy and uses it to move protons across the membrane out of the cell.

BBa_J70306
BBa_J70306 Version 1 (Component)
Similar to S03621, except PsrI sites (some portions of B0010, B0012) removed

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

BBa_K239004
BBa_K239004 Version 1 (Component)
Part of narGP promoter, activated by Fnr during oxygen deficiency

BBa_K176017
BBa_K176017 Version 1 (Component)
pCI(R0051)(lambda CI-)->RBS+GFP+T

BBa_K182100
BBa_K182100 Version 1 (Component)
Intermediate Part consisting of Lambda cI with Reporter Gene and double Terminators

BBa_K175014
BBa_K175014 Version 1 (Component)
PLambda in+RBS+GFP+TT

BBa_K175024
BBa_K175024 Version 1 (Component)
PLacI+Key3c+TT+PLambdain+RBS+GFP+TT

BBa_K175025
BBa_K175025 Version 1 (Component)
PLacI+RBS+TetR+TT+PLambda+RBS+GFP+TT

BBa_K238006
BBa_K238006 Version 1 (Component)
Short version of vanillin synthesis

M13 -47
BBa_K176059 Version 1 (Component)
M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ

BBa_K238012
BBa_K238012 Version 1 (Component)
short version II of vanillin synthesis

BBa_K237002
BBa_K237002 Version 1 (Component)
Constitutive RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

BBa_K237004
BBa_K237004 Version 1 (Component)
Constitutive sRIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

BBa_K176088
BBa_K176088 Version 1 (Component)
Reverse sequencing primer binds to the middle of luxR (VRluxR)

BBa_K182004
BBa_K182004 Version 1 (Component)
Lambda CI promoter (BBa_R0051) with a point mutation in -35 region.

BBa_K196006
BBa_K196006 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid) + RBS (Elowitz 1999)

BBa_K196007
BBa_K196007 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid) + RFP + RBS (GluColi project)

Plamda-GFP
BBa_I763011 Version 1 (Component)
promoter lambda (cI regulated) with GFP (+LVA) reporter

BBa_E2030
BBa_E2030 Version 1 (Component)
enhanced version of EYFP, yeast-optimized

VR3
BBa_K176047 Version 1 (Component)
Reverse primer VR3 (bind to K176037), member of VR3~VR12

VR4
BBa_K176048 Version 1 (Component)
Reverse primer VR4 (bind to K176038), member of VR3~VR12

VR5
BBa_K176049 Version 1 (Component)
Reverse primer VR5 (bind to K176039), member of VR3~VR12

VR6
BBa_K176050 Version 1 (Component)
Reverse primer VR6 (bind to K176040), member of VR3~VR12

VR7
BBa_K176051 Version 1 (Component)
Reverse primer VR7 (bind to K176041), member of VR3~VR12

VR8
BBa_K176052 Version 1 (Component)
Reverse primer VR8 (bind to K176042), member of VR3~VR12

VR9
BBa_K176053 Version 1 (Component)
Reverse primer VR9 (bind to K176043), member of VR3~VR12

VR10
BBa_K176054 Version 1 (Component)
Reverse primer VR10 (bind to K176044), member of VR3~VR12

VR11
BBa_K176055 Version 1 (Component)
Reverse primer VR11 (bind to K176045), member of VR3~VR12

VR12
BBa_K176056 Version 1 (Component)
Reverse primer VR12 (bind to K176046), member of VR3~VR12

BBa_K200016
BBa_K200016 Version 1 (Component)
Temperature Sensitive Lambda cI Repressor