BBa_M31113
BBa_M31113 Version 1 (Component)
Part of Gene III, M13 phage, and terminator of gVIII.

BBa_K1444014
BBa_K1444014 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - pTetR x2

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

BBa_K519023
BBa_K519023 Version 1 (Component)
Promoter(High) and RBS with PduP1~18 fused fMT

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K542006
BBa_K542006 Version 1 (Component)
pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K09100
BBa_K09100 Version 1 (Component)
Receiver for AHL and Outputs GFP when AHL is present

BBa_K1231003
BBa_K1231003 Version 1 (Component)
Asr-64-Lpp-RBS-GFP is a dual-state construct

BBa_K1989037
BBa_K1989037 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

pTetR-RFP-
BBa_I763040 Version 1 (Component)
RFP regulated by TetR and GFP regulated by pLac

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS

BBa_K1657006
BBa_K1657006 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

BBa_M11402
BBa_M11402 Version 1 (Component)
5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K584008
BBa_K584008 Version 1 (Component)
Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_K603002
BBa_K603002 Version 1 (Component)
same as BBa_K603000 except in pSB1C3 vector

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_K1479002
BBa_K1479002 Version 1 (Component)
Testing twins mechanism, same as BBa_B0014, no practical purpose

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

pLacIQ1-RB
BBa_K193406 Version 1 (Component)
the coding is the same as BBa_193404 the back bone is changed onto pSB6A1