BBa_K1463807BBa_K1463807 Version 1 (Component)RFP under J23112 promoter
BBa_K187019BBa_K187019 Version 1 (Component)RFP in pAB, Biobytes plasmid
BBa_K2041010BBa_K2041010 Version 1 (Component)plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T
BBa_K092300BBa_K092300 Version 1 (Component)RFP controlled by a TetR promoter
BBa_K783068BBa_K783068 Version 1 (Component)J23103 RFP composite part
BBa_K876035BBa_K876035 Version 1 (Component)pLuxR-LasI-RBS-RFP
BBa_K206004BBa_K206004 Version 1 (Component)RFP generator for testing pBAD wt
BBa_K2036027BBa_K2036027 Version 1 (Component)pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
BBa_S04522BBa_S04522 Version 1 (Component)LuxI with RFP reporter
BBa_K809108BBa_K809108 Version 1 (Component)Efficiency test of Q0255 Terminator
BBa_K091230BBa_K091230 Version 1 (Component)cI promoter+RBS+RFP+TT
BBa_I731012BBa_I731012 Version 1 (Component)Constitutive luxR + luxpR-controlled RFP
BBa_K337088BBa_K337088 Version 1 (Component)Fragment 7 of wt AAV6
BBa_K337060BBa_K337060 Version 1 (Component)Fragment 3 of wt AAV1
BBa_K1413042BBa_K1413042 Version 1 (Component)OriVR6Kgamma origin of replication (ori) 2
BBa_J107029BBa_J107029 Version 1 (Component)J23101 measurement system (with RFP)
BBa_J107032BBa_J107032 Version 1 (Component)F2620 measurement system (with RFP)
BBa_K1673110BBa_K1673110 Version 1 (Component)Standard RFP with IPTG Promoter
BBa_J107028BBa_J107028 Version 1 (Component)J23100 measurement system (with RFP)
BBa_K272001BBa_K272001 Version 1 (Component)Constitutive Expression Cassette for RFP
BBa_K315035BBa_K315035 Version 1 (Component)pTet-loxBri(F)-RBS-RFP-lox2272
BBa_K315025BBa_K315025 Version 1 (Component)pTet-loxBri(F)-RBS-RFP-loxN
BBa_K315018BBa_K315018 Version 1 (Component)pTet-loxP-RBS-RFP-loxBri(F)
BBa_K577001BBa_K577001 Version 1 (Component)RFP Coding Device (Not Repressible)
BBa_J01106BBa_J01106 Version 1 (Component)TEST [pLacLam][StrRBS][RFP][DblTerm]
BBa_K1845000BBa_K1845000 Version 1 (Component)RFP Reporter/Screening plasmid intermediate
T25 domainBBa_K1088056 Version 1 (Component)T25 domain of CyaA from Bordetella pertussis
BBa_S03681BBa_S03681 Version 1 (Component)(-2,-1) RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-TB-RBS<sub>rev</sub>-HixC : pLac<sub>rev</sub>-HixC
BBa_S03688BBa_S03688 Version 1 (Component)(-1,-2) RFP<sub>rev</sub>RBS<sub>rev</sub>-HixC-pLac<sub>rev</sub>-HixC : TB-RBS<sub>rev</sub>-HixC
BBa_I715041BBa_I715041 Version 1 (Component)pLac-RBS-RFP-RBS-GFP
BBa_K1197008BBa_K1197008 Version 1 (Component)Pveg (K823003) + RBS (SpoVG) + RFP + DT
BBa_I732747BBa_I732747 Version 1 (Component)P_U073O27+D062NUL + Double Reporters (lacZa+RFP)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_M31786BBa_M31786 Version 1 (Component)1st half of Gene III- preBamHI cut
BBa_K648103BBa_K648103 Version 1 (Component)RecA with mutation of Arg 243
CsgDBBa_K1019001 Version 1 (Component)CsgD: positive regulator of curlin genes
BBa_K648102BBa_K648102 Version 1 (Component)RecA with mutation of Lys 286
BBa_J119075BBa_J119075 Version 1 (Component)pT7-RBS-RFP-pLacI-RBS-T7RNAP
BBa_K1491033BBa_K1491033 Version 1 (Component)T7 promoter-RBS-RFP-T7 Terminator
BBa_J133000BBa_J133000 Version 1 (Component)RFP Reporter Cassette B0032.E1010.B0015
BBa_J133001BBa_J133001 Version 1 (Component)RFP Reporter Cassette B0034.E1010.B0015
BBa_J01098BBa_J01098 Version 1 (Component)TEST [Str RBS][RFP][Dbl Term]
BBa_K315023BBa_K315023 Version 1 (Component)pTet-loxBri(F)-RBS-RFP-loxBri(F)
BBa_K1114003BBa_K1114003 Version 1 (Component)The MoClo format of BBa_J23103 with AB fusion sites.
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
BBa_J01119BBa_J01119 Version 1 (Component)TEST [pBad/araC][StrRBS][RFP][DblTerm]
BBa_K546547BBa_K546547 Version 1 (Component)Constitutive (tetR repressible) LacI and RFP expression
BBa_K264028BBa_K264028 Version 1 (Component)Constitutive promoter BBa_J23102/RFP reporter BBa_J06702