BBa_K609004
BBa_K609004 Version 1 (Component)
A Nisin resistance gene nisI for Streptococcus thermophilus

BBa_K609003
BBa_K609003 Version 1 (Component)
A Nisin resistance gene nisI for Lactobacillus bulgaricus

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_I12223
BBa_I12223 Version 1 (Component)
TetR-4C protein generator

BBa_K091177
BBa_K091177 Version 1 (Component)
pMnt/tetR-RBS-GFP-TT-pBAD-RBS-tetR-TT

BBa_K091175
BBa_K091175 Version 1 (Component)
pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT

BBa_I739102
BBa_I739102 Version 1 (Component)
Double Promoter (cI, negative / TetR, negative)

BBa_K165063
BBa_K165063 Version 1 (Component)
pRS306 yeast shuttle vector, URA3 selection

BBa_K165061
BBa_K165061 Version 1 (Component)
pRS304* yeast shuttle vector, TRP1 selection

BBa_K1351023
BBa_K1351023 Version 1 (Component)
upp cassette as counter-selection marker

BBa_K165062
BBa_K165062 Version 1 (Component)
pRS305 yeast shuttle vector, LEU2 selection

BBa_K1456025
BBa_K1456025 Version 1 (Component)
tetR-ODD-VP16

BBa_K133048
BBa_K133048 Version 1 (Component)
TetR-UreB-Histop

BBa_K332031
BBa_K332031 Version 1 (Component)
Constitutive promoter + 37℃ induced RBS + tetR + double terminator

BBa_K1473018
BBa_K1473018 Version 1 (Component)
gRNA for tetR 2

BBa_K613022
BBa_K613022 Version 1 (Component)
TetR E37A P39K mutant

BBa_K1473021
BBa_K1473021 Version 1 (Component)
gRNA for tetR 5

BBa_K1473017
BBa_K1473017 Version 1 (Component)
gRNA for tetR 1

BBa_K613018
BBa_K613018 Version 1 (Component)
TetR Y42F K108E mutant

BBa_K1936002
BBa_K1936002 Version 1 (Component)
tetR-Pif6 for yeast

BBa_K1473020
BBa_K1473020 Version 1 (Component)
gRNA for tetR 4

BBa_K1473019
BBa_K1473019 Version 1 (Component)
gRNA for tetR 3

BBa_K2066121
BBa_K2066121 Version 1 (Component)
Synthetic Enhancer Project: 2X TetO Binding Cassette(55aS) + NRII + TetR + sfGFP on UNS

BBa_K2066122
BBa_K2066122 Version 1 (Component)
Synthetic Enhancer Project: 3X TetO Binding Cassette(52S) + NRII + TetR + sfGFP on UNS

BBa_K1076001
BBa_K1076001 Version 1 (Component)
Flanking genes for FadR deletion

BBa_J119368
BBa_J119368 Version 1 (Component)
Ptac mutant -40 G Deletion

BBa_K1124103
BBa_K1124103 Version 1 (Component)
clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only)

BBa_K1366022
BBa_K1366022 Version 1 (Component)
Genetic construct for msbB gen deletion

BBa_K1366021
BBa_K1366021 Version 1 (Component)
Genetic construct for lpp gen deletion

BBa_J13011
BBa_J13011 Version 1 (Component)
TetR repressed Cph8

BBa_J24810
BBa_J24810 Version 1 (Component)
R0040 promoter + pbrt (lead resistance gene)

BBa_K1486027
BBa_K1486027 Version 1 (Component)
R.reniformis luciferase + ADH1 terminator + Kanamycin resistance

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K1475903
BBa_K1475903 Version 1 (Component)
tetR with LVA and terminator

BBa_K336015
BBa_K336015 Version 1 (Component)
p(tetR)+RBS+α-amylase+stop

BBa_K1673010
BBa_K1673010 Version 1 (Component)
Partially UV-Resistant RFP

iGEM 2019 Distribution
iGEM2019Distribution_collection Version 1 (Collection)
Distribution of parts for the 2019 iGEM competition

iGEM 2018 Distribution
iGEM2018Distribution_collection Version 1 (Collection)
Distribution of parts for the 2018 iGEM competition

BBa_K577892
BBa_K577892 Version 1 (Component)
TetR Expression Plasmid

BBa_K611009
BBa_K611009 Version 1 (Component)
TetR Characterization Construct

BBa_K145113
BBa_K145113 Version 1 (Component)
Constitutive PoPS -> TetR

BBa_K561005
BBa_K561005 Version 1 (Component)
Pvgb+bglX+tetR

BBa_K577891
BBa_K577891 Version 1 (Component)
TetR Expression Plasmid

BBa_K081018
BBa_K081018 Version 1 (Component)
tetR constitutive generator

iGEM Distributions
iGEMDistributions_collection Version 1 (Collection)
Part distributions for the iGEM competition

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K1520003
BBa_K1520003 Version 1 (Component)
Pcons2-rbs-luxR-Ter

BBa_I739106
BBa_I739106 Version 1 (Component)
Double Promoter (TetR, negative / P22 cII, negative)

BBa_K678041
BBa_K678041 Version 1 (Component)
pyrG-DR cassette, for selection of Aspergillus nidulans