BBa_K541800BBa_K541800 Version 1 (Component)B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli
BBa_K782048BBa_K782048 Version 1 (Component)HindIII/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vector
BBa_K782049BBa_K782049 Version 1 (Component)HindIII/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vector
BBa_K782047BBa_K782047 Version 1 (Component)HindIII/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vector
BBa_K2092004BBa_K2092004 Version 1 (Component)alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
BBa_K1558000BBa_K1558000 Version 1 (Component)Clostridial shuttle vector
BBa_K799025BBa_K799025 Version 1 (Component)pSB1C3 yGG ORF acceptor vector
BBa_K299509BBa_K299509 Version 1 (Component)Expression Vector pT7+B0034
BBa_K799026BBa_K799026 Version 1 (Component)pSB1C3 yGG transcriptional terminator acceptor vector
BBa_J95039BBa_J95039 Version 1 (Component)plasmid vector for Rhodobacter sphaeroides
BBa_J01120BBa_J01120 Version 1 (Component)Sacrafical OnRFP Kan vector
BBa_K112995BBa_K112995 Version 1 (Component)BBb1 assembly vector - C/A
BBa_K1351040BBa_K1351040 Version 1 (Component)pBS0K<i>Pspac</i>, an IPTG-inducible replicative expression vector for
BBa_J72117BBa_J72117 Version 1 (Component)BBb High copy entry vector, pBca1256
BBa_K541715BBa_K541715 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide
BBa_K106693BBa_K106693 Version 1 (Component)AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
BBa_K1114400BBa_K1114400 Version 1 (Component)This is a MoClo level 0 destination vector.
BBa_K1033204BBa_K1033204 Version 1 (Component)pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
BBa_K802003BBa_K802003 Version 1 (Component)Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.