BBa_K590016
BBa_K590016 Version 1 (Component)
sfGFP_mamI_pGA1C3

BBa_K523017
BBa_K523017 Version 1 (Component)
Plac + RBS + rcsA

BBa_K523018
BBa_K523018 Version 1 (Component)
Plac + RBS + C. fimi β-glucosidase

BBa_K638301
BBa_K638301 Version 1 (Component)
Arabinose inducible Reflectin A1-sfGFP fusion generator

BBa_K649303
BBa_K649303 Version 1 (Component)
PlacIQ-RBS-ispS

BBa_K638403
BBa_K638403 Version 1 (Component)
Arabinose inducible TorA-Reflectin A1-sfGFP generator

BBa_K738007
BBa_K738007 Version 1 (Component)
FB, Split GFP C-terminal domain

BBa_K887070
BBa_K887070 Version 1 (Component)
Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC

BBa_K887071
BBa_K887071 Version 1 (Component)
Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC

BBa_J72203
BBa_J72203 Version 1 (Component)
kanamycin resistant split R6K vector

BBa_J72204
BBa_J72204 Version 1 (Component)
ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT

BBa_J72205
BBa_J72205 Version 1 (Component)
ampicillin resistant split ColE2 vector

BBa_K741020
BBa_K741020 Version 1 (Component)
plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T

BBa_K741021
BBa_K741021 Version 1 (Component)
plac-RBS-cI-T-pRM-anticro-T

BBa_J72206
BBa_J72206 Version 1 (Component)
ampicillin resistant split R6K vector

BBa_K821010
BBa_K821010 Version 1 (Component)
PLacI+Native RBS+RFP+Native RBS+GFP+Terminator

BBa_K821011
BBa_K821011 Version 1 (Component)
PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157005
BBa_K157005 Version 1 (Component)
Split-Cerulean-cCFP

BBa_K157006
BBa_K157006 Version 1 (Component)
Split-Cerulean-nCFP

BBa_K157007
BBa_K157007 Version 1 (Component)
Split-Venus-cYFP

BBa_K157008
BBa_K157008 Version 1 (Component)
Split-Venus-nYFP

linker
BBa_K157009 Version 1 (Component)
Split fluorophore linker; Freiburg standard

Tm-Split-L
BBa_K157020 Version 1 (Component)
Tm-Split-Luciferase1/2

SP-AntiNIP
BBa_K157030 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157031 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

SP-Lipocal
BBa_K157038 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

SP-Lipocal
BBa_K157039 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

Tm-Splitlu
BBa_K157021 Version 1 (Component)
Tm-Splitluciferase2/2

BBa_K142205
BBa_K142205 Version 1 (Component)
Plac + RBS + SceI + double terminator

BBa_I715039
BBa_I715039 Version 1 (Component)
pLac-RBS-RFP

BBa_K116633
BBa_K116633 Version 1 (Component)
pLac + RBS + CII + T

BBa_K116638
BBa_K116638 Version 1 (Component)
pLac + RBS + LuxI + T

BBa_K116639
BBa_K116639 Version 1 (Component)
Tuner: pLac + RBS + CIIICd + T

BBa_K199013
BBa_K199013 Version 1 (Component)
pLacIQ1-RBS32-GFP-TT

BBa_K199049
BBa_K199049 Version 1 (Component)
pLac+RBS+CAT+TT on pSB1AK3

BBa_K199050
BBa_K199050 Version 1 (Component)
pLacIQ-RBS-RFP

BBa_K175016
BBa_K175016 Version 1 (Component)
PLacI+RBS

BBa_K175020
BBa_K175020 Version 1 (Component)
PLacI+RBS+TetR+TT

BBa_K175024
BBa_K175024 Version 1 (Component)
PLacI+Key3c+TT+PLambdain+RBS+GFP+TT

BBa_K175025
BBa_K175025 Version 1 (Component)
PLacI+RBS+TetR+TT+PLambda+RBS+GFP+TT

BBa_K199064
BBa_K199064 Version 1 (Component)
pLac-RBS-CCCUC-RFP

BBa_K199065
BBa_K199065 Version 1 (Component)
pLac-RBS-CCAUC-RFP

BBa_K199066
BBa_K199066 Version 1 (Component)
pLac-RBS-CCACU-RFP

BBa_K199067
BBa_K199067 Version 1 (Component)
pLac-RBS-CUAGU-RFP

BBa_K199068
BBa_K199068 Version 1 (Component)
pLac-RBS-CGGUC-RFP

pLacI::Tet
BBa_K257004 Version 1 (Component)
pLacI + RBS-TetR

BBa_K235004
BBa_K235004 Version 1 (Component)
[R0010][B0034] (pLac+RBS)

BBa_K199084
BBa_K199084 Version 1 (Component)
pLac-RBS-AGGAC-RFP

BBa_K199085
BBa_K199085 Version 1 (Component)
pLac-RBS-CUACC-RFP