BBa_K292010
BBa_K292010 Version 1 (Component)
D protein from the Lambda phage

BBa_K758002
BBa_K758002 Version 1 (Component)
GAL4, this protein binds the UAS

BBa_I741008
BBa_I741008 Version 1 (Component)
brings xylose into the cell (doesn

HE Promote
BBa_K1539055 Version 1 (Component)
HE Promoter Primer

HE RBS
BBa_K1539021 Version 1 (Component)
HE RBS Primer

BBa_J100198
BBa_J100198 Version 1 (Component)
J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes

BBa_J64715
BBa_J64715 Version 1 (Component)
Constituive expression of LasR

BBa_J64716
BBa_J64716 Version 1 (Component)
Constituive expression of RHLR

YFP_SPtom2
BBa_K809318 Version 1 (Component)
YFP + signal peptide of Tom22

Linker-FOS
BBa_K1616015 Version 1 (Component)
Linker FOS of 17 aa

Linker-JUN
BBa_K1616018 Version 1 (Component)
Linker JUN of 5 aa

BBa_I723126
BBa_I723126 Version 1 (Component)
This is the short description

BBa_K758000
BBa_K758000 Version 1 (Component)
UAS , this is the enhancer . And this enhancer is used to identify the protein localization .

BBa_K2004001
BBa_K2004001 Version 1 (Component)
Construct of CDO

BBa_I751500
BBa_I751500 Version 1 (Component)
pcI (for positive control of pcI-lux hybrid promoter)

BBa_K2144002
BBa_K2144002 Version 1 (Component)
ESP capable of inhibiting biofilm

BBa_J58121
BBa_J58121 Version 1 (Component)
Detector of OmpR-P concentrations

BBa_J11013
BBa_J11013 Version 1 (Component)
tet regulated control of CI(ts)

BBa_J06650
BBa_J06650 Version 1 (Component)
Test of BBa_R0051

RBS-pcyA
BBa_K193402 Version 1 (Component)
RBS is located upstream of pcyA

T18 domain
BBa_K1638004 Version 1 (Component)
T18 domain of CyaA from Bordetella pertussis

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

CBPa-BpA
BBa_K2053001 Version 1 (Component)
Fusion cellulose-binding domain of CBP-B domain of protein A

BBa_K1937007
BBa_K1937007 Version 1 (Component)
AF-A : operon of antifungal peptides

BBa_K1365111
BBa_K1365111 Version 1 (Component)
dspB with signal sequence of USP45

BBa_K374002
BBa_K374002 Version 1 (Component)
the Gifsy-2 phage lysogenic repressor gtgR

BBa_K374000
BBa_K374000 Version 1 (Component)
the Gifsy-1 phage lysogenic repressor gogR

YFP_SPtom4
BBa_K809316 Version 1 (Component)
YFP + signal peptide of Tom40

BBa_K1660005
BBa_K1660005 Version 1 (Component)
RFP controlled by the PompR promoter

BBa_J23080
BBa_J23080 Version 1 (Component)
b0034 derived RBS to match spacing of J01122

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1001758
BBa_K1001758 Version 1 (Component)
AraC coding upstream of Lux promoter upstream of GFP

BBa_I11013
BBa_I11013 Version 1 (Component)
Modification of biobricks part BBa_B0015

Strong+Imm
BBa_K320006 Version 1 (Component)
Constitutive expression of Immunotoxin

BBa_K1058003
BBa_K1058003 Version 1 (Component)
Promoter of penicillinase blaZ as well as promoter of blaR1

BBa_K189061
BBa_K189061 Version 1 (Component)
Gp16 of Bacteriophage Mu

BBa_K835002
BBa_K835002 Version 1 (Component)
cytoplasmic region of tar

BBa_K189065
BBa_K189065 Version 1 (Component)
E18 of Bacteriophage Mu

BBa_K189068
BBa_K189068 Version 1 (Component)
C of Bacteriophage Mu

BBa_J100020
BBa_J100020 Version 1 (Component)
Second Half of AspC

BBa_K189052
BBa_K189052 Version 1 (Component)
GP7 of Bacteriophage Mu

BBa_K189050
BBa_K189050 Version 1 (Component)
Kil of Bacteriophage Mu

BBa_M30050
BBa_M30050 Version 1 (Component)
Refactoring of M13K07 genome

BBa_K189075
BBa_K189075 Version 1 (Component)
Gp28 of Bacteriophage Mu

BBa_K189056
BBa_K189056 Version 1 (Component)
Gp11 of Bacteriophage Mu

BBa_K189062
BBa_K189062 Version 1 (Component)
Gp16 of Bacteriophage Mu

BBa_K189057
BBa_K189057 Version 1 (Component)
Gp12 of Bacteriophage Mu

BBa_J61052
BBa_J61052 Version 1 (Component)
[neuS1] of K1 ORF

BBa_K189051
BBa_K189051 Version 1 (Component)
Gp6 of Bacteriophage Mu