| Types | DnaRegion
|
| Roles | Measurement
engineered_region
|
| Sequences | BBa_I22000_sequence (Version 1)
|
Description
This construct is a scaffold device for measuring RBS strength via GFP fluorescence. The device contains a ccdB expression cassette (BBa_P1010) in place of an RBS. Digestion with AvrII and SapI allows for the insertion of any RBS sequence via Heather Keller's alternative RBS assembly method, which allows for blunt cloning of an RBS upstream of a coding sequence.
Notes
Due to the presence of ccdB (BBa_P1010) this device can only be grown in DB3.1 or other permissive strains.
Source
This is a composite part formed from subparts available in the registry.
