BBa_I715050

BBa_I715050 Version 1

Component

This part has been discontinued.

Please consider using BBa_I715049 as a replacement.

Source:
http://parts.igem.org/Part:BBa_I715050
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amanda Valencia
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:07:50

Hin + pT7 -> RFP1 + GFP1 medium initial orientation



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Device

    Sequences BBa_I715050_sequence (Version 1)

    Description

    Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC. The corresponding part without Hin does not glow. With Hin, most colonies do not glow, but green and red colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.

    Notes

    None

    Source

    Ligation of existing parts

    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    isReplacedBy
    BBa_I715049/1
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I715050/1
     

    Attachments

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