Types | DnaRegion
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Roles | Composite
engineered_region
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Sequences | BBa_K081004_sequence (Version 1)
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Description
This composite part is the union of:
*a PoPS in -> cI, lasR out device and
*a Plambda-regulated luxR protein generator with Plux downstream.
It is a module of the following genetic demultiplexer (demux):
{|align="center"
|[[Image:Pv_demux_final.png|500px]]
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|[[Image:pv_demuxregistry_interactions.png|500px]]
|}
where:
*PA, PS and PB are three promoters that can be ACTIVATED by the three generic elements A, S and B respectively;
*GOI is the gene of interest that is the output of the system.
In particular, K081004 is the Selector.
People who want to use the genetic mux have to assemble:
*K081000 under the desired regulation for Channel 0 (for example the generic promoter PA);
*K081001 under the desired regulation for Channel 1 (for example the generic promoter PB);
*K081002 under the desired regulation for Selector (for example the generic promoter PS);
*the desired protein generator downstream of K081000 and K081001 (for example the generic RBS-GOI-T generator);
Notes
We used BioBrick Standard Assembly.
Source
a