Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: alexandra bouaziz
Date created: 2008-10-24 11:00:00
Date modified: 2015-05-08 01:10:02
flgA promoter - YFP Tripart (LVA+)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K136012_sequence (Version 1) |
Description
flgA promotor followed by its RBSPromoter of flgA gene from E. coli flagella
flgA is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).
Its promoter is consitutively repressed.
It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
flgA promotor is here followed by YFP tripart (containing a LVA tail).
Notes
flgA was digested by EcoRI and SpeI (insert) and cloned into the J61002 plasmid containing YFP tripart LVA.Source
flgA promotor comes from E. coli K12 strain MG1655.| Access | Instance | Definition |
| public public public public public | BBa_K136006 BBa_B0034 BBa_E0032 BBa_B0012 BBa_B0011 | BBa_K136006 BBa_B0034 YFP BBa_B0012 BBa_B0011 |