Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:04
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1361006_sequence (Version 1) |
Description
This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of Pbad promotor. CsgBtrunc is different from native CsgB from the deletion of C-terminal amino acid from 133 to 155 that it cannot attach cell outer membrane. And CsgBtrunc itself is highly aggregative in the culture.This part is designed to be used for immediate generate of large amount of curli fiber after adding of the inducer and a stronger promoter for CsgA was chosen to parallel with BBa_K1361002.
This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
Notes
The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.Source
CsgAC and CsgBtrunc were collected by PCR from genome of E coli DH5alpha respectively.| Access | Instance | Definition |
| public public public public public public | BBa_K206000 BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K880005 BBa_K1361998 | pBAD BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K880005 BBa_K1361998 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K880005 BBa_K1361998 | 1,130 139,150 157,555 564,692 701,755 762,1608 | pBAD BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K880005 BBa_K1361998 |