Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-18 02:54:25
AND GATE-input_AHL+green light
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1886010_sequence (Version 1) |
Description
The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by AHL, pLuxR promoter activates the expression of T7 RNA polymerase (T7 ptag) which contains two amber mutations. After being induced by green light, PcpcG2 promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, when AHL and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.Notes
PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.Source
This part is a combination of AHL input and Green light input. The subparts of this part is from iGEM Distribution Kits.| Access | Instance | Definition |
| public public public public public public public | BBa_K592003 BBa_K228001 BBa_B0015 BBa_R0062 BBa_B0033 BBa_K228000 BBa_B0015 | BBa_K592003 BBa_K228001 BBa_B0015 lux pR BBa_B0033 BBa_K228000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592003 BBa_K228001 BBa_B0015 BBa_R0062 BBa_B0033 BBa_K228000 BBa_B0015 | 1,238 247,382 391,519 528,582 591,601 608,3280 3289,3417 | BBa_K592003 BBa_K228001 BBa_B0015 lux pR BBa_B0033 BBa_K228000 BBa_B0015 |