Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-19 09:11:42
AND GATE-input_red light+IPTG
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1886014_sequence (Version 1) |
Description
The circuit to receive input signals is composed of AND GATE, plac promoter and light-controlled promoter. After being induced by IPTG, plac promoter activates the expression of T7 RNA polymerase (T7 ptag) which contains two amber mutations. The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CL protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CL protein(for more information, see the part"PompC-NOT Gate"). Therefore, when IPTG and red light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.Notes
PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.Source
This part is a combination of IPTG input and Red light input. The subparts of this part are from iGEM Distribution Kits.| Access | Instance | Definition |
| public public public public public public public | BBa_K1145005 BBa_K228001 BBa_B0015 BBa_R0010 BBa_B0033 BBa_K228000 BBa_B0015 | Pλ BBa_K228001 BBa_B0015 LacI BBa_B0033 BBa_K228000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1145005 BBa_K228001 BBa_B0015 BBa_R0010 BBa_B0033 BBa_K228000 BBa_B0015 | 1,68 77,212 221,349 358,557 566,576 583,3255 3264,3392 | Pλ BBa_K228001 BBa_B0015 LacI BBa_B0033 BBa_K228000 BBa_B0015 |