Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-19 10:50:40
AND GATE-input_ara+IPTG
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1886016_sequence (Version 1) |
Description
This is the input part of AND GATE. After being induced by L-arabinose, pbad promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by IPTG, plac promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.Notes
PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.Source
This part is a combination of IPTG input and ara input. The subparts of this part are from iGEM Distribution Kits.| Access | Instance | Definition |
| public public public public public public public public public public | BBa_I13453 BBa_K228001 BBa_B0015 BBa_R0010 BBa_B0033 BBa_K228000 BBa_B0015 BBa_K1321338 BBa_K895006 BBa_B0015 | BBa_I13453 BBa_K228001 BBa_B0015 LacI BBa_B0033 BBa_K228000 BBa_B0015 BBa_K1321338 BBa_K895006 BBa_B0015 |