Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team TU Munich 2011
Date created: 2011-08-31 11:00:00
Date modified: 2015-05-08 01:12:43
Optogenetical AND-Gate red/blue light
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K568001_sequence (Version 1) |
Description
This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD. The blue light sensor induces the expression of a mRNA coding for a T7-polymerase with an amber mutation, that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor is induced. Thus, only cells which receive both signals produce the desired substances.Notes
We used YcgF/E as blue light sensor and Cph8 as red light sensor. We expect that this assembly will be the one with the lowest amount of unspecific gene expression which could occur due to overlapping of the absorption spectrums of the sensory domains.Source
designed from existing Biobricks| Access | Instance | Definition |
| public public public public public public public public | BBa_K322123 BBa_K322124 BBa_R0082 BBa_K228001 BBa_B0015 BBa_K238013 BBa_J44001 BBa_K228000 | BBa_K322123 BBa_K322124 OmpR BBa_K228001 BBa_B0015 BLp BBa_J44001 BBa_K228000 |