Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alişan Kayab??len
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:33
T4 Lysis Device under control of IPTG
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K843008_sequence (Version 1) |
Description
By using this composite part, it is possible to keep living of bacteria under control of IPTG. This part contains a Kill Switch lysis machine designed by iGEM08 UC Berkeley team, with a cI regulated promoter. Also, there is a cI coding region with a LacI regulated promoter.By this way, in a medium containing IPTG, LacI will be inhibited by IPTG. Therefore, LacI regulated promoter will be active and cI protein will be produced. As a result, cI protein will inhibit cI regulated promoter activity. So, kill switch part (T4 Lysis Device) will not work. In contrast, in the absence of IPTG, LacI inhibits its promoter, and so cI will not be produced. Therefore, cI regulated promoter will be active and T4 Lysis Device take bacteria to the lysis.
As a result, this composite part has a big potential to be used as a safety device.
Notes
Since our project has a potential to be used as a biofilter, safety is a big issue. To create a very safe part, we thought that bacteria should be alive only in our system. If any escape from system occurs, bacteria should kill themselves. Thus, we thought to make their lives depending on an easy-found medium compound, IPTG.Source
All parts used for this device is present in parts registry.| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_R0051 BBa_B0034 BBa_K112805 BBa_K112806 BBa_B0010 BBa_B0012 BBa_J23116 BBa_K112907 BBa_B0010 BBa_R0010 BBa_B0034 BBa_C0051 | cI lam BBa_B0034 BBa_K112805 BBa_K112806 BBa_B0010 BBa_B0012 BBa_J23116 BBa_K112907 BBa_B0010 LacI BBa_B0034 cI lam |