Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rada Jumroon
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:41
Blue Light Sensor, TetR Inverter and sfGFP Output
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K892800_sequence (Version 1) |
Description
pLac I - LovTAP - Term - pTrp - TetR - Term - pTetO - sfGFP - TermPart BBa_892800 is an improvement on part
BBa_K191003. It fixes a frameshift deletion in LovTAP and includes
the inverter and sfGFP readout on a single plasmid. The hybrid protein
LovTAP has a light-sensitive domain (Lov: Light, oxygen, voltage) and
a regulatory domain (part of the E. coli Trp repressor). The fused
protein allows transmission of the conformational change induced by
light (on the light-sensitive domain) to the DNA-binding domain,
therefore resulting in an increase or decrease of the regulatory
domain's affinity for its DNA binding site. The activation of the
LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm
For more details, please consult the section of LovTAP system on team
EPF-Lausanne wiki, or the App page (include link here) on the
University of Washington wiki.
Usage and Biology
In order to be able to test the efficiency of the fused protein
LovTAP, its expression is induced by IPTG (Due to the LacI-sensitive
promoter). The readout of the system is also included on the plasmid.
The TetR driven by the TrpR promoter acts as an inverter, while the
super-folder GFP (sfGFP) driven by the TetO promoter is the ultimate
readout. Also available in the registry are the two parts: BBa_K191004
and BBa_K191005.
Notes
Whatever.Source
BBa_K191003 from team iGEM09_EPF-Lausanne.| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_R0010 BBa_B0030 BBa_K892801 BBa_B0010 BBa_B0012 BBa_K191007 BBa_B0033 BBa_C0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_I746916 | LacI BBa_B0030 BBa_K892801 BBa_B0010 BBa_B0012 TrpR BBa_B0033 tetR BBa_B0010 BBa_B0012 p(tetR) BBa_I746916 |