BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_J92001
BBa_J92001 Version 1 (Component)
Lead Remover and Reporter Device

BBa_J107021
BBa_J107021 Version 1 (Component)
aTc sensor (J23106 promoter) with GFP

BBa_K1484005
BBa_K1484005 Version 1 (Component)
AmilCP chromoprotein in BBa and MoClo standard

BBa_K1113001
BBa_K1113001 Version 1 (Component)
pSB4K5 with a promoter and RBS

BBa_K1463771
BBa_K1463771 Version 1 (Component)
MotA and MotB under J23112 promoter

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

RBS-TetR-T
BBa_K079007 Version 1 (Component)
TetR protein with terminator and RBS

BBa_K1332003
BBa_K1332003 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon

BBa_M31909
BBa_M31909 Version 1 (Component)
Part of Gene 3 and the BamHI Restriction Site

BBa_K2097001
BBa_K2097001 Version 1 (Component)
Blue chromoprotein with strong promoter and RBS

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K1172506
BBa_K1172506 Version 1 (Component)
oprF with strong Promoter and strong RBS

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K1463753
BBa_K1463753 Version 1 (Component)
MotB and B0032 RBS under J23103 promoter

BBa_K1463703
BBa_K1463703 Version 1 (Component)
MotA and B0032 RBS under J23103 promoter

BBa_J14465
BBa_J14465 Version 1 (Component)
Composite part comprised of J13002 and I13401

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_I732091
BBa_I732091 Version 1 (Component)
Double Repoters (LacZ-alpha and GFP-AAV)

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_J14457
BBa_J14457 Version 1 (Component)
Composite part comprised of R0010 and E0422

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3

BBa_K1676091
BBa_K1676091 Version 1 (Component)
Mutant 15 of LDH and wildtype LDP

BBa_K1676086
BBa_K1676086 Version 1 (Component)
Mutant 10 of LDH and wildtype LDP

BBa_K1676080
BBa_K1676080 Version 1 (Component)
Mutant 4 of LDH and wildtype LDP

BBa_J14467
BBa_J14467 Version 1 (Component)
Composite part comprised of I6032 and I13601

BBa_J14466
BBa_J14466 Version 1 (Component)
Composite part comprised of I6031 and I13600

BBa_K1444011
BBa_K1444011 Version 1 (Component)
Composite promoter and consensus B. subtilis RBS - pTetR

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

BBa_K1088058
BBa_K1088058 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site

BBa_K1638006
BBa_K1638006 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site

BBa_M50056
BBa_M50056 Version 1 (Component)
PETase double mutant I208V and R90A full plasmid information

BBa_K648123
BBa_K648123 Version 1 (Component)
RecA with mutation of Lys 286 and Arg 243

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

PLac-LacY-
BBa_I763013 Version 1 (Component)
LacY and cI coding device switched on by IPTG

BBa_M31113
BBa_M31113 Version 1 (Component)
Part of Gene III, M13 phage, and terminator of gVIII.

BBa_K1444014
BBa_K1444014 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - pTetR x2

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

BBa_K519023
BBa_K519023 Version 1 (Component)
Promoter(High) and RBS with PduP1~18 fused fMT

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K542006
BBa_K542006 Version 1 (Component)
pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K09100
BBa_K09100 Version 1 (Component)
Receiver for AHL and Outputs GFP when AHL is present

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

pTetR-RFP-
BBa_I763040 Version 1 (Component)
RFP regulated by TetR and GFP regulated by pLac

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast