BBa_K1084004
BBa_K1084004 Version 1 (Component)
promoter 4

BBa_K1916011
BBa_K1916011 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916022
BBa_K1916022 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916005
BBa_K1916005 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916008
BBa_K1916008 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916010
BBa_K1916010 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916023
BBa_K1916023 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916017
BBa_K1916017 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916015
BBa_K1916015 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916020
BBa_K1916020 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916019
BBa_K1916019 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916009
BBa_K1916009 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916001
BBa_K1916001 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916018
BBa_K1916018 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K398206
BBa_K398206 Version 1 (Component)
AlnA generator (IPTG inducible)

BBa_K879034
BBa_K879034 Version 1 (Component)
Promoter 4

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

BBa_K801092
BBa_K801092 Version 1 (Component)
4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1923010
BBa_K1923010 Version 1 (Component)
Backup 4

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_K1423006
BBa_K1423006 Version 1 (Component)
Arsenic Inducible Banana Odor Generator

BBa_K1491016
BBa_K1491016 Version 1 (Component)
Lac Inducible Codon Optimized KillerRed

BBa_K1491018
BBa_K1491018 Version 1 (Component)
Lac Inducible Codon Optimized Supernova

BBa_K1319042
BBa_K1319042 Version 1 (Component)
IPTG inducible iLOV

BBa_K1075027
BBa_K1075027 Version 1 (Component)
SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

scFv
BBa_K1933002 Version 1 (Component)
anti-Norovirus GII.4 scFv

BBa_K731400
BBa_K731400 Version 1 (Component)
IPTG inducible Cysteine desulfhydrase (CysDes)

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K1473004
BBa_K1473004 Version 1 (Component)
gRNA 4

BBa_K2116050
BBa_K2116050 Version 1 (Component)
Pconst_esa(4)

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K1470002
BBa_K1470002 Version 1 (Component)
Galactose-induced gene 4 DNA binding domain (Gal4DBD)