BBa_J23012BBa_J23012 Version 1 (Component)Spectinomycin R open-reading-frame basic part
BBa_K1212001BBa_K1212001 Version 1 (Component)Theophylline Riboswitch (Clone E)
BBa_K176133BBa_K176133 Version 1 (Component)Repressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPS
BBa_K176230BBa_K176230 Version 1 (Component)High input score automatic selector (with reporter): PoPS->GFP->K176229
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_I13261BBa_I13261 Version 1 (Component)Lux Receiver (I13263 with reversed part order)
AntiFreezeBBa_K193200 Version 1 (Component)TmTHP is one of Anti-Freeze Protein
BBa_J24696BBa_J24696 Version 1 (Component)18. pTetR - BBa_C0080
BBa_J24712BBa_J24712 Version 1 (Component)32. M RBS - LacI - S RBS - EYFP(+LVA) - terminator
BBa_K2057006BBa_K2057006 Version 1 (Component)Expression of gfp under the regulation of promoter BtI-BtII
BBa_K079050BBa_K079050 Version 1 (Component)GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator
BBa_K577894BBa_K577894 Version 1 (Component)TetR repressed YFP
BBa_K1820006BBa_K1820006 Version 1 (Component)sfGFP(Bs) + terminator
BBa_K1820007BBa_K1820007 Version 1 (Component)mCherry(Lr) + terminator
BBa_K176237BBa_K176237 Version 1 (Component)Repressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPS
BBa_M36129BBa_M36129 Version 1 (Component)PP7 aptamer
BBa_I13538BBa_I13538 Version 1 (Component)Two promoter screening plasmid v1.1
BBa_I13539BBa_I13539 Version 1 (Component)Two promoter screening plasmid v1.2
BBa_K896903BBa_K896903 Version 1 (Component)VEGF of homo
BBa_K1362173BBa_K1362173 Version 1 (Component)Split sfGFP reconstitution N-terminal splicing half
BBa_J24691BBa_J24691 Version 1 (Component)13. pLacI - BBa_J24680
BBa_J24694BBa_J24694 Version 1 (Component)16. pTetR - BBa_J24680
BBa_K137114BBa_K137114 Version 1 (Component)AHL induced production of RcsA
BBa_K1913019BBa_K1913019 Version 1 (Component)Guanine riboswitch BS-yxjA
rbs + sam5BBa_K238007 Version 1 (Component)Sam5 gene with RBS
BBa_K1151012BBa_K1151012 Version 1 (Component)LuxI nickel regulated
BBa_K577893BBa_K577893 Version 1 (Component)TetR repressed GFP
BBa_I759019BBa_I759019 Version 1 (Component)cis7-repressed, tet-regulated YFP
BBa_K1725309BBa_K1725309 Version 1 (Component)Member of the RBS library - derived from BBa_B0032
BBa_K1725307BBa_K1725307 Version 1 (Component)Member of the RBS library - derived from BBa_B0032
BBa_K1725305BBa_K1725305 Version 1 (Component)Member of the RBS library - derived from BBa_B0032
BBa_K199116BBa_K199116 Version 1 (Component)RBS-RFP-TT-pLac-RBS-LuxR-TT
BBa_K1212000BBa_K1212000 Version 1 (Component)Theophylline Riboswitch (Clone 8.1*)
BBa_J70506BBa_J70506 Version 1 (Component)Mesoplasma florum origin of replication
BBa_J70503BBa_J70503 Version 1 (Component)Mesoplasma florum origin of replication
BBa_J70505BBa_J70505 Version 1 (Component)Mesoplasma florum origin of replication
BBa_J70509BBa_J70509 Version 1 (Component)Mesoplasma florum origin of replication
BBa_J70508BBa_J70508 Version 1 (Component)Mesoplasma florum origin of replication
miRts lucBBa_J176025 Version 1 (Component)miRts luc
BBa_K089006BBa_K089006 Version 1 (Component)-663 to Tc start site of phaC
BBa_K089005BBa_K089005 Version 1 (Component)-35 to Tc start site of phaC
BBa_K1879000BBa_K1879000 Version 1 (Component)CDS of N-CBZ cleavage enzyme
BBa_I751100BBa_I751100 Version 1 (Component)J54140 delPr
BBa_K876011BBa_K876011 Version 1 (Component)pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
BBa_J24697BBa_J24697 Version 1 (Component)19. pBAD - BBa_J24683
BBa_K1689004BBa_K1689004 Version 1 (Component)Coding sequence of Nluc398-FRB
BBa_J24709BBa_J24709 Version 1 (Component)29. M RBS - TetR - S RBS - ECFP (+LVA) - terminator
pSC101BBa_I50040 Version 1 (Component)nonfunctional pSC101 origin of replication
BBa_K808034BBa_K808034 Version 1 (Component)araC-Pbad regulated terephtalate periplasmatic binding proteine of the tripartite transporter family