Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_J70575
BBa_J70575 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold v 5.0, no BsaXI, V169I in E0040

lux pL
BBa_R0063 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pL)<br>

tetR
BBa_Q04400 Version 1 (Component)
QPI (B0034.C0040.B0015.R0040)

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_J70550
BBa_J70550 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_J70568
BBa_J70568 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold v 3.0

BBa_E0044
BBa_E0044 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

BBa_J70571
BBa_J70571 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold v 4.0

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

BBa_I7101
BBa_I7101 Version 1 (Component)
R0040.B0032.E0040.B0015

BsaI ->
BBa_K1362425 Version 1 (Component)
BsaI restriction site for RFC[???] cloning (in part in prefix)

BBa_J70399
BBa_J70399 Version 1 (Component)
from J04450 {0,5:15,10}

BBa_J70428
BBa_J70428 Version 1 (Component)
{7,12:15,10} red version

BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)

lux pR
BBa_R1062 Version 1 (Component)
Promoter, Standard (luxR and HSL regulated -- lux pR)<br>

BBa_K325903
BBa_K325903 Version 1 (Component)
luxEG <BR><i>V. Fischeri<BR>(E. coli optimised)</i>

BBa_J70567
BBa_J70567 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold

rhIR
BBa_C0171 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)

BBa_J70587
BBa_J70587 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold (R0040-B0034-E1010-B0010-B0012)

BBa_J70607
BBa_J70607 Version 1 (Component)
R0010 + B0034 (-BsaXI site in R0010)

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites

BBa_J85202
BBa_J85202 Version 1 (Component)
R0040.B0034.E0040

BsaI ->
BBa_K1362424 Version 1 (Component)
BsaI restriction site for RFC[105] cloning

BBa_S03518
BBa_S03518 Version 1 (Component)
B0034:C0040

BBa_J70588
BBa_J70588 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold (reverse R0040-B0034-E1010-B0010-B0012)

BBa_E5503
BBa_E5503 Version 1 (Component)
R0040.B0030.E0040

BBa_E5504
BBa_E5504 Version 1 (Component)
R0040.B0032.E0040

BBa_J70400
BBa_J70400 Version 1 (Component)
from J04430, {0,5:15,10}

BBa_E5500
BBa_E5500 Version 1 (Component)
B0030.E0040

aiiA
BBa_C0160 Version 1 (Component)
autoinducer inactivation enzyme aiiA (no LVA)