Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0015BBa_B0015 Version 1 (Component)double terminator (B0010-B0012)
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
ATGATG Version 1 (Component) BBa_B0014BBa_B0014 Version 1 (Component)double terminator (B0012-B0011)
RFC105 ZBBa_K1362421 Version 1 (Component)C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
BBa_B0025BBa_B0025 Version 1 (Component)double terminator (B0015), reversed
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_K2066527BBa_K2066527 Version 1 (Component)B0034 with scar TACTAG
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
BBa_K1051003BBa_K1051003 Version 1 (Component)Stop codon free GFP in RFC[23] standard
BBa_K1051000BBa_K1051000 Version 1 (Component)Stop codon free RFP in RFC[23] standard
BBa_M36084BBa_M36084 Version 1 (Component)Histidine Tag and Stop Codon
BBa_K1332002BBa_K1332002 Version 1 (Component)Histidine tag (8 AA) and RFP (without stop codon)
BBa_K1321340BBa_K1321340 Version 1 (Component)Double CBD (dCBD) with N-terminal linker
BBa_K1162009BBa_K1162009 Version 1 (Component)10x-Histidine (10x-His) Tag with Met (ATG) For N-terminal purification
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
BBa_K1051009BBa_K1051009 Version 1 (Component)e.coli terminator ilvGEDA_T with stop codon
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_B0024BBa_B0024 Version 1 (Component)double terminator (B0012-B0011), reversed
BBa_B0017BBa_B0017 Version 1 (Component)double terminator (B0010-B0010)
BBa_M36117BBa_M36117 Version 1 (Component)Stop Codon
BBa_K1051004BBa_K1051004 Version 1 (Component)Stop codon free mOrange in RFC[23] standard
BBa_K809601BBa_K809601 Version 1 (Component)GAL promoter (kozak) without start codon ATG
HisTagBBa_J18909 Version 1 (Component)His tag (6xHis; E. coli - optimized)
HABBa_I712008 Version 1 (Component)HA affinity tag
NLSBBa_J176008 Version 1 (Component)NLS
BBa_K208014BBa_K208014 Version 1 (Component)GFP (Silver Fusion)/Double Terminator
BBa_J70594BBa_J70594 Version 1 (Component)RFC12 TAATAA Tail Domain
BBa_K1051053BBa_K1051053 Version 1 (Component)K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)
BBa_K1162012BBa_K1162012 Version 1 (Component)GFP with 10x His Tag
BBa_K105012BBa_K105012 Version 1 (Component)10 aa flexible protein domain linker
PESTBBa_J176018 Version 1 (Component)PEST
BBa_K549019BBa_K549019 Version 1 (Component)10x His-tag
BBa_J64101BBa_J64101 Version 1 (Component)ColE1 biobrick plasmid with backward terminator
BBa_K1319007BBa_K1319007 Version 1 (Component)6xHis tag with double stop codon
linkerBBa_K133132 Version 1 (Component)8 aa protein domain linker