Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
mChBBa_J176005 Version 1 (Component)mCh
fshPCDBBa_J176002 Version 1 (Component)fshPCD
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
VP64BBa_J176013 Version 1 (Component)VP64
PLflexBBa_J176040 Version 1 (Component)PLflex
BBa_K1941000BBa_K1941000 Version 1 (Component)PCP fused to Mxi1 domain
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K1462503BBa_K1462503 Version 1 (Component)SH3 domain + Linker
BBa_K165006BBa_K165006 Version 1 (Component)Zif268-HIV DNA-binding domain
BBa_K300002BBa_K300002 Version 1 (Component)Phasin (PhaP) - head domain
Fok_aBBa_K243000 Version 1 (Component)Protein domain (active) of the restriction endonuclease FokI
RFC105 CCBBa_K1362419 Version 1 (Component)C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC
Gal4DBBBa_J176020 Version 1 (Component)Gal4 DB
BBa_J70594BBa_J70594 Version 1 (Component)RFC12 TAATAA Tail Domain
preSCsiteBBa_J18919 Version 1 (Component)preScission protease cleavage site (E. coli-optimized)
OmpABBa_K103006 Version 1 (Component)OmpA outer membrane protein A fused to linker; displays proteins on cell surface
BBa_K1462502BBa_K1462502 Version 1 (Component)PDZ domain + Linker
SPzim17BBa_K809201 Version 1 (Component)signal peptide of ZIM17
mCherryBBa_K165004 Version 1 (Component)mCherry, yeast optimized for fusion
BBa_K1321002BBa_K1321002 Version 1 (Component)Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25
BBa_K1321003BBa_K1321003 Version 1 (Component)Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
NpuDnaE(N)BBa_K1362400 Version 1 (Component)NpuDnaE N-Intein cloning piece
BBa_K1423000BBa_K1423000 Version 1 (Component)BCLA N-terminal Domain
BBa_K1321092BBa_K1321092 Version 1 (Component)Phytochelatin (PC) EC20 fused to CBDcex
BBa_K1321094BBa_K1321094 Version 1 (Component)CBDclos fused to Phytochelatin (PC) EC20
Sin3BBa_K165003 Version 1 (Component)Sin3 Repression Domain
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
BBa_K747095BBa_K747095 Version 1 (Component)TAL-Protein_TT6_Direpeat
AmCyanBBa_J176022 Version 1 (Component)AmCyan
RFC105 NCBBa_K1362417 Version 1 (Component)C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC
BBa_K1638016BBa_K1638016 Version 1 (Component)8 aa linker without BamHI restriction site and TEV recognition site
BBa_K1462501BBa_K1462501 Version 1 (Component)GBD domian + Linker