Cas9
Cas9 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_K2019003
BBa_K2019003 Version 1 (Component)
Periplasm Directed saCas9 via TorA Signaling Sequence (TorA-saCas9)

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_K2019005
BBa_K2019005 Version 1 (Component)
Periplasm Directed saCas9 via Ycdo Signaling Sequence (Ycdo-saCas9)

BBa_K2019004
BBa_K2019004 Version 1 (Component)
Periplasm Directed saCas9 via DsbA Signaling Sequence (DsbA-saCas9)

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

scar
BBa_K112999 Version 1 (Component)
BBb1 scar sequence

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

MorA
BBa_K1615000 Version 1 (Component)
Morphine-6-dehydrogenase RFC25

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

BBa_I746916
BBa_I746916 Version 1 (Component)
superfolder GFP coding sequence

BBa_K1150000
BBa_K1150000 Version 1 (Component)
dCas9

BBa_R0085
BBa_R0085 Version 1 (Component)
T7 Consensus Promoter Sequence

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_K208005
BBa_K208005 Version 1 (Component)
TorA Signal Peptide - Silver Fusion Compatible

BBa_K1378023
BBa_K1378023 Version 1 (Component)
TorA-MlrA

BBa_K797002
BBa_K797002 Version 1 (Component)
TorA signal with RBS

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_K1978002
BBa_K1978002 Version 1 (Component)
TorA-RFP

BBa_K130002
BBa_K130002 Version 1 (Component)
torA export

BBa_K1978000
BBa_K1978000 Version 1 (Component)
TorA-BtuF