Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

LasR+PAI
BBa_R0079 Version 1 (Component)
Promoter (LasR & PAI regulated)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K1375024
BBa_K1375024 Version 1 (Component)
Prhl/las +RBS

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_K1375019
BBa_K1375019 Version 1 (Component)
Prhl/las +RBS+eforRed

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

eforRed
BBa_K592012 Version 1 (Component)
eforRed, red chromoprotein

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

RhI
BBa_I14017 Version 1 (Component)
P(Rhl)

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

BBa_K914003
BBa_K914003 Version 1 (Component)
L-rhamnose-inducible promoter (pRha)

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

lasI
BBa_C0178 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)

BBa_K1375020
BBa_K1375020 Version 1 (Component)
Prhl/las +RBS+amilGFP

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_K1375002
BBa_K1375002 Version 1 (Component)
PRhl/Las+RBS+GFP+DT

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)