Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

TEV
BBa_I712077 Version 1 (Component)
TEV protease (N-terminal half) - entered sequence may be wrong

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_K1321340
BBa_K1321340 Version 1 (Component)
Double CBD (dCBD) with N-terminal linker

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

scar
BBa_K112999 Version 1 (Component)
BBb1 scar sequence

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

BBa_K1159101
BBa_K1159101 Version 1 (Component)
N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

BBa_I746916
BBa_I746916 Version 1 (Component)
superfolder GFP coding sequence

BBa_I712078
BBa_I712078 Version 1 (Component)
TEV protease (C-terminal half)

BBa_K249008
BBa_K249008 Version 1 (Component)
YFP with N-terminal Arganine Tag

LacI
BBa_K143033 Version 1 (Component)
LacI (Lva^-, N-terminal deletion) regulatory protein

GFP1
BBa_I715019 Version 1 (Component)
Amino Half of GFP (aka GFP1)

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_R0085
BBa_R0085 Version 1 (Component)
T7 Consensus Promoter Sequence

BBa_K1159102
BBa_K1159102 Version 1 (Component)
C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

GFP2
BBa_I715020 Version 1 (Component)
Carboxyl Half of GFP (aka GFP2)

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_K1423000
BBa_K1423000 Version 1 (Component)
BCLA N-terminal Domain

BBa_K1362173
BBa_K1362173 Version 1 (Component)
Split sfGFP reconstitution N-terminal splicing half

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

RFC105 NN
BBa_K1362415 Version 1 (Component)
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN