Cas9
Cas9 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ShortLinke
BBa_K243004 Version 1 (Component)
Short Linker (Gly-Gly-Ser-Gly)

LongLinker
BBa_K243006 Version 1 (Component)
Long Linker (Gly-Gly-Ser-Gly)x3

MiddleLink
BBa_K243005 Version 1 (Component)
Middle Linker ( Gly-Gly-Ser-Gly)x2

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

BBa_K1797015
BBa_K1797015 Version 1 (Component)
dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Bxb1 gp35

BBa_K1797016
BBa_K1797016 Version 1 (Component)
dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Flp

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

RFC105 CN
BBa_K1362418 Version 1 (Component)
N-terminal GG-linker overhang <u>GGTG</u>=Gly+1/3Gly RFC[105] overhang CN

RFC105 NC
BBa_K1362417 Version 1 (Component)
C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC

scar
BBa_K112999 Version 1 (Component)
BBb1 scar sequence

Bxb1_Int
BBa_K907000 Version 1 (Component)
Mycobacterium Phage Bxb1 gp35, DNA integrase

BBa_I766336
BBa_I766336 Version 1 (Component)
Ser-Gly-Ser-Gly-Ser ING2 PH Domain

BBa_I766234
BBa_I766234 Version 1 (Component)
Ser-Gly-Ser-Gly-Ser-Ent5p-ENTH PH Domain

BBa_I766231
BBa_I766231 Version 1 (Component)
Ser-Gly-Ser-Gly-Ser FYVE-EEA1 PH Domain

BBa_I766232
BBa_I766232 Version 1 (Component)
Ser-Gly-Ser-Gly-Ser-PLCdelta PH Domain

BBa_K165007
BBa_K165007 Version 1 (Component)
Gli-1 DNA-binding domain

BBa_K197029
BBa_K197029 Version 1 (Component)
gly-ser-repeats

BBa_K641005
BBa_K641005 Version 1 (Component)
ToxR with nhe1 and gly-ser linkers

Gly+1/3Leu
BBa_K1362478 Version 1 (Component)
Gly+1/3Leu

BBa_K656006
BBa_K656006 Version 1 (Component)
IPTG Reporter Using Cohesin-Gly-Ser-Gly-Cohesin

BBa_K259008
BBa_K259008 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BBa_K259009
BBa_K259009 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BioScaffol
BBa_K259004 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BioScaffol
BBa_K259002 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

2/3Gly
BBa_K1362467 Version 1 (Component)
other 2/3Gly

Gly3
BBa_K1362438 Version 1 (Component)
GGG linker

2/3Gly
BBa_K1362464 Version 1 (Component)
2/3Gly

BBa_K1508003
BBa_K1508003 Version 1 (Component)
IIA(Glc) + double terminator

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

GLF
BBa_K558005 Version 1 (Component)
Zymomonas mobilis glucose facilitator gene

BBa_K223025
BBa_K223025 Version 1 (Component)
HlyR + HlyC + Hly B + Hly D

GlF
BBa_K901008 Version 1 (Component)
Glucose Facilitator (Glf) from <i>Zymonas mobilis</i>

BBa_K1150000
BBa_K1150000 Version 1 (Component)
dCas9

BBa_K1508002
BBa_K1508002 Version 1 (Component)
IIA(Glc) - E.coli glucose-specific phosphotransferase enzyme IIA

BBa_K1469003
BBa_K1469003 Version 1 (Component)
glk

RFC105 NN*
BBa_K1362416 Version 1 (Component)
N-terminal non-splicing overhang GGCT=Gly+1/3Leu|Ile RFC[105] overhang NN*

LytC-Gly-E
BBa_K316045 Version 1 (Component)
LytC - Glycine Linker - Elastase Cleavage Site - AIP - His Tag