Cas9
Cas9 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

GFP
GFP Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S

HYG
Hygromicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

NEO (G418/Neomicin Resistance)
Neomicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

mCherry
mCherry Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

amdS
amdS Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

APP1
GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

ACT1
GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

Cre
BBa_J61047 Version 1 (Component)
Cre DNA recombinase

BBa_K1179015
BBa_K1179015 Version 1 (Component)
A U6_gRNA(Cr9) plasmid which produces Cr9 targeting guide RNA's for Cas9

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_I13507
BBa_I13507 Version 1 (Component)
Screening plasmid intermediate

BBa_K786033
BBa_K786033 Version 1 (Component)
cascade proteins, which consists of casA, casB, casC, casD, casE

BBa_K648028
BBa_K648028 Version 1 (Component)
Cro, Lamda Repressor which activates the lytic cycle

Pcar
BBa_K861171 Version 1 (Component)
Pcar, synthetic promoter repressed by CRP

CRP
BBa_J58112 Version 1 (Component)
CRP protein

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_I759002
BBa_I759002 Version 1 (Component)
pTet-spacer-cro

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_I13504
BBa_I13504 Version 1 (Component)
Screening plasmid intermediate

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A