BBa_K1074016
BBa_K1074016 Version 1 (Component)
RBS for Pctc

BBa_K1994022
BBa_K1994022 Version 1 (Component)
Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette

PgsiB
BBa_K143011 Version 1 (Component)
Promoter gsiB for B. subtilis

input-lacI
BBa_S03334 Version 1 (Component)
Constitutive lacI-production to block cI production.

BBa_K1362005
BBa_K1362005 Version 1 (Component)
Sortase A non-circularization construct (with Smt3 and His6)

BBa_K786003
BBa_K786003 Version 1 (Component)
Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light

BBa_K805015
BBa_K805015 Version 1 (Component)
CsgD, the master regulator of biofilm formation

BBa_04450*
BBa_K1362505 Version 1 (Component)
BBa_J04450 with a mutation

BBa_K1606091
BBa_K1606091 Version 1 (Component)
Gluten Updated m-cherry reporter device

BBa_K1189000
BBa_K1189000 Version 1 (Component)
TALE-A with a his tag under a lacI promoter

Ptet-ChiXm
BBa_K564020 Version 1 (Component)
Mutated regulatory device based on sRNA.

BBa_J72200
BBa_J72200 Version 1 (Component)
hlyE expresion cassette

BBa_K1716002
BBa_K1716002 Version 1 (Component)
NahR Biosensor for detection of acetylsalicylic acid/aspirin with blue chromoprotein reporter

BBa_I8023
BBa_I8023 Version 1 (Component)
Booperator test: Inverter NOR biasing in polycistronic design

BBa_I8022
BBa_I8022 Version 1 (Component)
Booperator test: Inverter NOR biasing in monocistronic design

nick
BBa_K1479014 Version 1 (Component)
Just for Test

THI3
BBa_J45009 Version 1 (Component)
ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

BBa_K284019
BBa_K284019 Version 1 (Component)
Lactate Permease constitutive expression

BBa_K299510
BBa_K299510 Version 1 (Component)
Expression Vector pT7+J6100

BBa_K1357006
BBa_K1357006 Version 1 (Component)
Manganese Peroxidase Expression Vector

BBa_J329052
BBa_J329052 Version 1 (Component)
Medium AiiA Expression Part

BBa_K1400003
BBa_K1400003 Version 1 (Component)
pTre(4) Single input tet responsive promoter

BBa_K1400005
BBa_K1400005 Version 1 (Component)
pTRE(2) Single input tetr responsive promoter

BBa_K116501
BBa_K116501 Version 1 (Component)
RpaA(regulator of phycobilisome-associated)

BBa_M50020
BBa_M50020 Version 1 (Component)
Promoter for GolS + GFP

BBa_J09266
BBa_J09266 Version 1 (Component)
constitutive GFP (AAV+) device, medium RBS

BBa_K863201
BBa_K863201 Version 1 (Component)
3' UTR site of alcohol oxidase 1 gene (aox1)

BBa_J13014
BBa_J13014 Version 1 (Component)
LacI repressed light responsive system

BBa_J13015
BBa_J13015 Version 1 (Component)
TetR repressed light responsive system

BBa_K313013
BBa_K313013 Version 1 (Component)
loading sequence of RNA phage MS2 (reverse)

BBa_I2011
BBa_I2011 Version 1 (Component)
B_Receiver Device (R1075, B0034.C0071.B0015)

BBa_K1583062
BBa_K1583062 Version 1 (Component)
Scar in CsgA_Mfp3

BBa_K1583063
BBa_K1583063 Version 1 (Component)
Scar in CsgA_Mfp3

BBa_K316019
BBa_K316019 Version 1 (Component)
Reverse strand coding ComE responsive promoter

BBa_K1614002
BBa_K1614002 Version 1 (Component)
transcription cassette of BBF RFC 110

xynA-PDZli
BBa_K1602035 Version 1 (Component)
Inducible generator of RBS-xynA-PDZlig

BBa_K2139001
BBa_K2139001 Version 1 (Component)
Coding Sequence of Endo5a

BBa_K1779203
BBa_K1779203 Version 1 (Component)
A gene from magnetotactic bacteria which associated with the formation of the magnetosome.

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

BBa_K1633006
BBa_K1633006 Version 1 (Component)
MOR siRNA-4 (siRNA for mouse Mu opioid receptor)

BBa_K1149006
BBa_K1149006 Version 1 (Component)
pelB-pulA-flag expression

BBa_K309022
BBa_K309022 Version 1 (Component)
pOdr-10::eCFP::unc-54 3'UTR (for use in C. elegans chassis)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

test
BBa_K1479015 Version 1 (Component)
Just for test

SPtom22
BBa_K809206 Version 1 (Component)
signal peptide of Tom22

BBa_T4002
BBa_T4002 Version 1 (Component)
luxC device regulated by lambda cl

BBa_K1351000
BBa_K1351000 Version 1 (Component)
beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC

BBa_K112114
BBa_K112114 Version 1 (Component)
a~Ligase!