BBa_K861042
BBa_K861042 Version 1 (Component)
FadA, gene for fatty acid degradation from <i>Salmonella enterica</i> LT2

BBa_J06701
BBa_J06701 Version 1 (Component)
mCherry with RBS and forward terminator

recAp-cheZ
BBa_K629010 Version 1 (Component)
recAp-cheZ-eGFP

BBa_K1796005
BBa_K1796005 Version 1 (Component)
Pnif+GFP reporter

BBa_J100080
BBa_J100080 Version 1 (Component)
Riboswitch and GFP

BBa_J119020
BBa_J119020 Version 1 (Component)
GFP-RBS-pT7

BBa_K649207
BBa_K649207 Version 1 (Component)
PlacIQ-lox2272-gfp

BBa_K1150033
BBa_K1150033 Version 1 (Component)
Constitutive GFP Reporter

BBa_K1555009
BBa_K1555009 Version 1 (Component)
GFP+copa+T7polymerase

BBa_K1461009
BBa_K1461009 Version 1 (Component)
GFP (AAV tag +)

BBa_J100201
BBa_J100201 Version 1 (Component)
RBS (BD24) + GFP

BBa_K1195007
BBa_K1195007 Version 1 (Component)
promoter ydiV + GFP

BBa_K1195008
BBa_K1195008 Version 1 (Component)
promoter FtSQAZ + GFP

BBa_K1615079
BBa_K1615079 Version 1 (Component)
CBDcenA+Linker fused to TVEL5 driven by LacI promoter

BBa_K1615036
BBa_K1615036 Version 1 (Component)
Linker+dCBD fused to MaoA driven by LacI promoter

BBa_K1615101
BBa_K1615101 Version 1 (Component)
CBDcenA+Linker fused to mRFP driven by LacI promoter

BBa_K1615037
BBa_K1615037 Version 1 (Component)
MaoA fused to Linker+dCBD driven by LacI promoter

BBa_K1615104
BBa_K1615104 Version 1 (Component)
mRFP fused to Linker+dCBD driven by LacI promoter

BBa_K1615060
BBa_K1615060 Version 1 (Component)
HerE fused to Linker+dCBD driven by LacI promoter

BBa_K1615103
BBa_K1615103 Version 1 (Component)
Linker+dCBD fused to mRFP driven by LacI promoter

BBa_K1615081
BBa_K1615081 Version 1 (Component)
Linker+dCBD fused to TVEL5 driven by LacI promoter

BBa_K1615012
BBa_K1615012 Version 1 (Component)
CBDcenA+Linker fused to MorA driven by LacI promoter

BBa_K1615014
BBa_K1615014 Version 1 (Component)
Linker+dCBD fused to MorA driven by LacI promoter

BBa_K1615034
BBa_K1615034 Version 1 (Component)
CBDcenA+Linker fused to MaoA driven by LacI promoter

BBa_K1615015
BBa_K1615015 Version 1 (Component)
MorA fused to Linker+dCBD driven by LacI promoter

BBa_K1758105
BBa_K1758105 Version 1 (Component)
mRFP generator under control of T7 promoter

BBa_J100283
BBa_J100283 Version 1 (Component)
rClone Red with RBS: Device for GGA Cloning and Testing RBS elements and Riboswitches

eGFP-PTS
BBa_K678017 Version 1 (Component)
eGFP-PTS-module

BBa_K2116019
BBa_K2116019 Version 1 (Component)
RBS designed for sfgfp

BBa_K1725346
BBa_K1725346 Version 1 (Component)
<i>luxC</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725349
BBa_K1725349 Version 1 (Component)
<i>luxE</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725347
BBa_K1725347 Version 1 (Component)
<i>luxG</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725348
BBa_K1725348 Version 1 (Component)
<i>luxD</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725345
BBa_K1725345 Version 1 (Component)
<i>luxB</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725344
BBa_K1725344 Version 1 (Component)
<i>luxA</i> with optimised RBS for expression in <i>E. coli</i>

eGFP-TS
BBa_K678008 Version 1 (Component)
eGFP-TS

BBa_K2144006
BBa_K2144006 Version 1 (Component)
Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter

BBa_K2144100
BBa_K2144100 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG-tag regulated by T7-promoter

BBa_K2144012
BBa_K2144012 Version 1 (Component)
Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter

BBa_K2144007
BBa_K2144007 Version 1 (Component)
Coding sequence for Defensin with His6 and LPXTG-tag regulated by T7-promoter

BBa_K091131
BBa_K091131 Version 1 (Component)
pLacIQ1 controlled GFP generator

BBa_K944002
BBa_K944002 Version 1 (Component)
Transcriptional Regulator for Cyanide Inducible Promoter

BBa_K1132042
BBa_K1132042 Version 1 (Component)
R1-pLac riboregulator switch with with pTET and pLac

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_J176091
BBa_J176091 Version 1 (Component)
HPK-10xGal4-CFP

BBa_K364146
BBa_K364146 Version 1 (Component)
Zinc Finger Protein CTG

BBa_K364123
BBa_K364123 Version 1 (Component)
Zinc Finger Protein GGC