BBa_S01379
BBa_S01379 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01663
BBa_S01663 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01660
BBa_S01660 Version 1 (Component)
Intermediate part from assembly 240

BBa_K511816
BBa_K511816 Version 1 (Component)
Inducible Blue Fluorescent Protein Generator (TRE-Tight-EBFP2) MammoBlock Device

BBa_K511815
BBa_K511815 Version 1 (Component)
Inducible Red Fluorescent Protein Generator (TRE-Tight-mKate) MammoBlock Device

BBa_S01898
BBa_S01898 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01381
BBa_S01381 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01830
BBa_S01830 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01845
BBa_S01845 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01380
BBa_S01380 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01377
BBa_S01377 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01842
BBa_S01842 Version 1 (Component)
Intermediate part from assembly 240

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_S01383
BBa_S01383 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01843
BBa_S01843 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01378
BBa_S01378 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01826
BBa_S01826 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01382
BBa_S01382 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01821
BBa_S01821 Version 1 (Component)
Intermediate part from assembly 240

BBa_K1983016
BBa_K1983016 Version 1 (Component)
PheP under constitutive promoter and tRNA-Phe under DH10B promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_S02001
BBa_S02001 Version 1 (Component)
Intermediate part from assembly 240

BBa_K1072016
BBa_K1072016 Version 1 (Component)
pGAL1 + Brho + EGFP + ADH1 Te

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

BBa_K619894
BBa_K619894 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K1447004
BBa_K1447004 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K1447005
BBa_K1447005 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K619895
BBa_K619895 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833002
BBa_K833002 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833006
BBa_K833006 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833008
BBa_K833008 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K1072013
BBa_K1072013 Version 1 (Component)
pGAL1+ Flag + odr-10 + GFP + ADH1 Te

BBa_K511817
BBa_K511817 Version 1 (Component)
Inducible Yellow Fluorescent Protein Generator (TRE-Tight-EYFP-FF4x4) MammoBlock Device

BBa_K511906
BBa_K511906 Version 1 (Component)
Inducible CI434-VP16 Transactivator Generator (TRE-Tight-CI434-VP16) MammoBlock Device

BBa_K511910
BBa_K511910 Version 1 (Component)
Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) MammoBlock Device

BBa_K511908
BBa_K511908 Version 1 (Component)
Inducible Gal4-VP16 Transactivator Generator (TRE-Tight-Gal4-VP16) MammoBlock Device

BBa_K511814
BBa_K511814 Version 1 (Component)
Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device

BBa_K511907
BBa_K511907 Version 1 (Component)
Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device

BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K1179078
BBa_K1179078 Version 1 (Component)
TRE-tight

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1766008
BBa_K1766008 Version 1 (Component)
EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.

BBa_K1195004
BBa_K1195004 Version 1 (Component)
Cro from Lamda phage with shine delgarno added.

BBa_K1766014
BBa_K1766014 Version 1 (Component)
EnvZ osmoregulatory histidine kinase from <i>E.coli.</i>

BBa_K1072014
BBa_K1072014 Version 1 (Component)
pGAL1+ Flag + Brho + odr-10 + GFP + ADH1 Te

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K1947017
BBa_K1947017 Version 1 (Component)
We verify the expression effect of Mms13.