BBa_K568005
BBa_K568005 Version 1 (Component)
Intermediate optogenetical AND-Gate without T7ptag

BBa_K568006
BBa_K568006 Version 1 (Component)
Intermediate synthetic part of optogenetical AND-Gate

BBa_K607023
BBa_K607023 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 0.07RBS

BBa_K607040
BBa_K607040 Version 1 (Component)
reverse pBad/araC promoter

BBa_K607024
BBa_K607024 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 0.3RBS

BBa_K607025
BBa_K607025 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 0.6RBS

BBa_K607026
BBa_K607026 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 1.0RBS

BBa_K607027
BBa_K607027 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 0.07RBS

BBa_K607028
BBa_K607028 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 0.3RBS

BBa_K607029
BBa_K607029 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 0.6RBS

BBa_K607030
BBa_K607030 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 1.0RBS

BBa_K607031
BBa_K607031 Version 1 (Component)
PlasB_LasR_antipBad/araC with 0.07RBS

BBa_K607032
BBa_K607032 Version 1 (Component)
PlasB_LasR_antipBad/araC with 0.3RBS

BBa_K607033
BBa_K607033 Version 1 (Component)
PlasB_LasR_antipBad/araC with 0.6RBS

BBa_K607034
BBa_K607034 Version 1 (Component)
PlasB_LasR_antipBad/araC with 1.0RBS

BBa_K611009
BBa_K611009 Version 1 (Component)
TetR Characterization Construct

BBa_K611010
BBa_K611010 Version 1 (Component)
LacI Repressor Characterization Construct

BBa_K611011
BBa_K611011 Version 1 (Component)
cI lambda Repressor Characterization Construct

BBa_K611012
BBa_K611012 Version 1 (Component)
pTet Characterization Construct

BBa_K611013
BBa_K611013 Version 1 (Component)
pLacI Characterization Construct

BBa_K611014
BBa_K611014 Version 1 (Component)
cI Repressible Promoter Characterization Construct

BBa_K510016
BBa_K510016 Version 1 (Component)
pUC18R6KT-miniTn7BB-Gm-pBad/araC

BBa_K607036
BBa_K607036 Version 1 (Component)
pBAD/AraC generating GFP

BBa_K607037
BBa_K607037 Version 1 (Component)
pBAD/AraC generating cI transcription factor

AraC_TEV-F
BBa_K627009 Version 1 (Component)
Arabinose inducible TEV protease

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

BBa_K611018
BBa_K611018 Version 1 (Component)
Mutant Regulatory protein/promoter characterization plasmid

BBa_K581012
BBa_K581012 Version 1 (Component)
pqrr-rbs-T7ptag-terminator

BBa_K581013
BBa_K581013 Version 1 (Component)
lux repressible promoter-T7ptag-terminator

BBa_K581015
BBa_K581015 Version 1 (Component)
pBAD-supD-plux_inv-T7ptag

BBa_K764020
BBa_K764020 Version 1 (Component)
Inducible pBad/araC promoter + BBa_B0034

BBa_J72179
BBa_J72179 Version 1 (Component)
AraC-pBad

BBa_K914000
BBa_K914000 Version 1 (Component)
pLac-supD-T

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

BBa_K113007
BBa_K113007 Version 1 (Component)
pBad/araC->T7 polymerase

BBa_K113009
BBa_K113009 Version 1 (Component)
pBad/araC

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K136066
BBa_K136066 Version 1 (Component)
araC/pBad - EnvZ*

BBa_K177025
BBa_K177025 Version 1 (Component)
GFP under araC promotor

AraC O2
BBa_R0081 Version 1 (Component)
Inhibitor (AraC loop attachment with O2 site)

BBa_K233003
BBa_K233003 Version 1 (Component)
AND Gate 1

BBa_K233005
BBa_K233005 Version 1 (Component)
AND gate 2

BBa_K233006
BBa_K233006 Version 1 (Component)
AND Gate 3

BBa_K091223
BBa_K091223 Version 1 (Component)
AND Gate supporting construct