BBa_K1053120
BBa_K1053120 Version 1 (Component)
RNA scaffold containing HTLV-1 Rex peptide and λN peptide aptamer

BBa_K1216004
BBa_K1216004 Version 1 (Component)
β-Glucuronidase (gusA) from Bacillis Subtilis with TEV and poly-HIS tags

BBa_K1657005
BBa_K1657005 Version 1 (Component)
It is called BAG. It have the resistance to glyphosate and glufosinate

BBa_K1216006
BBa_K1216006 Version 1 (Component)
Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags

BBa_K598025
BBa_K598025 Version 1 (Component)
Bistable Switch with TPP Hammerhead Ribozyme 2.5 and Theophyline Riboswitch N8-3

BBa_K1657007
BBa_K1657007 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

BBa_K1104999
BBa_K1104999 Version 1 (Component)
This coding can make E. coli produce Chitinase-63, and degrade chitin.

RBS-HisTag
BBa_K1602022 Version 1 (Component)
Green fluorescent protein (GFP) with N-terminal His-Tag and RBS BBa_B0034

BBa_J04480
BBa_J04480 Version 1 (Component)
EYFP coding device with promoter, RBS, and terminator. No degradation tag.

BBa_I12226
BBa_I12226 Version 1 (Component)
Generator for wild-type TetR and lambda cI - Gal4 fusion protein

BY-Toggle
BBa_K1908000 Version 1 (Component)
Blue-Yellow fluorescent protein toggle switch using pLac and OmpC promoter.

BBa_K1613022
BBa_K1613022 Version 1 (Component)
BBa_K1613020,a composite part which has PmerT,B0032,RFP and B0015.

BBa_J58012
BBa_J58012 Version 1 (Component)
Promoter activated synergistically by cI and CRP with the reporter RFP

BBa_K1749000
BBa_K1749000 Version 1 (Component)
Cd sensitive promoter with Phi delta activator, PO promoter, and GFP

BBa_J33205
BBa_J33205 Version 1 (Component)
hybrid lambda PR-lac promoter, repressed by lambda cI and lacI, plus lacZ

BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

BBa_K543016
BBa_K543016 Version 1 (Component)
Bacteriophage Lysis Cassette S105, R, and Rz under Tet promoter (conjugative)

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K541029
BBa_K541029 Version 1 (Component)
Reflectin 1A (TAT-IPTG inducable) and LALF (TAT-IPTG inducable) protein for E.coli

BBa_K1465214
BBa_K1465214 Version 1 (Component)
RubisCO of Halothiobacillus neapolitanus and phosphoribulokinase A (prkA) under control of ptac

BBa_K1172566
BBa_K1172566 Version 1 (Component)
Combination of oprF under control of T7 promoter and gldA coding sequence

BBa_K195412
BBa_K195412 Version 1 (Component)
This part is composed of RBS,PelB,F10scFv and C-IgAP ,Term

BBa_K390029
BBa_K390029 Version 1 (Component)
GFP with 5'UTR and consensus RBS from Synechocystis sp. PCC 6803

BBa_K1175100
BBa_K1175100 Version 1 (Component)
BBa_K1175028 pBAD Promoter and SecI pump attached to Composite norvirus antibody BBa_K875004

BBa_K215010
BBa_K215010 Version 1 (Component)
pLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags on GFP

BBa_K1938013
BBa_K1938013 Version 1 (Component)
Chlorite dismutase with Constitutive Strong promoter, Strong RBS and TAT signal peptide

BBa_K390028
BBa_K390028 Version 1 (Component)
GFP with 5'UTR and native RBS from Synechocystis sp. PCC 6803

BBa_M31541
BBa_M31541 Version 1 (Component)
This is the M13K07 genome refactored between the Hap1 and Bam1 Sites

BBa_K2117005
BBa_K2117005 Version 1 (Component)
Device encoding TEF1 and hrGFP for expression in <i>Yarrowia lipolytica</i>

BBa_K1662000
BBa_K1662000 Version 1 (Component)
Construct 1 Bicistronic:PelB-CDA under tetR and GFP under P(Lac)IQ promoter

BBa_K1739003
BBa_K1739003 Version 1 (Component)
Sequence coding for Envirowire: Sup35NM with N-terminal CsgAss and C-terminal cytb562

BBa_K1711002
BBa_K1711002 Version 1 (Component)
yeVenus-PEST-TheoA; Coding sequence for destabilized YFP Venus and theophylline-sensitive aptazyme

BBa_J119315
BBa_J119315 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)

BBa_J70606
BBa_J70606 Version 1 (Component)
J70590 cut with BsaXI and with dead fusion inserts (J70556d-fi,ri) added

BBa_K2082006
BBa_K2082006 Version 1 (Component)
Nanobody library: Fusion proteins of Nanobody and omega subunit of RNA polymerase (rpoZ)

BBa_K1180002
BBa_K1180002 Version 1 (Component)
fusion protein of lamp 2b and RVG for brain targeting of the exosome

BBa_K2170114
BBa_K2170114 Version 1 (Component)
Intermediate of biotin binding receptor and secretory BirA (IRES IgKappa_BirA StrepDEL_PolyA) in RFC

BBa_K1045015
BBa_K1045015 Version 1 (Component)
RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240

BBa_K2082000
BBa_K2082000 Version 1 (Component)
Monobody: Constant regions and RFP (exchange with variable regions to create your library)

BBa_K1067000
BBa_K1067000 Version 1 (Component)
Periplasmic directed GFP SF with signal peptide TAT and RFP as background color

BBa_M302009
BBa_M302009 Version 1 (Component)
Region between HpaI site in gene II and BamHI site in gene III

BBa_K1456007
BBa_K1456007 Version 1 (Component)
Superoxide dismutase-1 (SOD-1) Forward primer with restriction site and kozak sequence

BBa_J64852
BBa_J64852 Version 1 (Component)
DNA stretch between OmpR sigma 70 promoter -10 site and start of transcription

P<sub>xyl<
BBa_K531002 Version 1 (Component)
P_xyl: Inducible promoter and RBS from <i>Caulobacter crescentus</i>

BBa_K2082005
BBa_K2082005 Version 1 (Component)
Monobody library: Fusion proteins of Monobody and omega subunit of RNA polymerase (rpoZ)

BBa_K1711001
BBa_K1711001 Version 1 (Component)
yeVenus-TheoA; coding sequence for enhanced yellow fluorescent protein and theophylline-sensative ap

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G

BBa_K863022
BBa_K863022 Version 1 (Component)
bhal laccase from Bacillus halodurans with constitutive promoter J23100, RBS and HIS tag

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K359005
BBa_K359005 Version 1 (Component)
Senses AIP and has RFP reporter (from Agr quorum sensing system)