BBa_K1114108BBa_K1114108 Version 1 (Component)Bicistronic design RBS 8, MoClo Format with BC fusion sites
BBa_K1523007BBa_K1523007 Version 1 (Component)A Device to reduce Cr(VI)
BBa_J119307BBa_J119307 Version 1 (Component)pSB1A2-BR-HS-A-RNA-I
BBa_J119312BBa_J119312 Version 1 (Component)pSB1A2-BR-HS-T-RNA-I
BBa_J119308BBa_J119308 Version 1 (Component)pSB1A2-BR-HS-C-RNA-I
BBa_K1058000BBa_K1058000 Version 1 (Component)Cr(VI) sensor with egfp(PchrB+chrB+egfp)
BBa_K1114104BBa_K1114104 Version 1 (Component)MoClo formatted version of B0034 with BC fusion sites
BBa_K1114103BBa_K1114103 Version 1 (Component)MoClo formatted version of B0033 with BC fusion sites
BBa_K1124123BBa_K1124123 Version 1 (Component)inverse PCR template for creating new sRNA (plambda-micC sRNA scaffold-terminator)
BBa_K325239BBa_K325239 Version 1 (Component)Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>
yjbC-P2BO_3429 Version 1 (Component) BBa_K783058BBa_K783058 Version 1 (Component)Level 0 MoClo Destination Vector with BC
BBa_K1114106BBa_K1114106 Version 1 (Component)Bicistronic Design RBS 1, MoClo Format with BC fusion sites
BBa_K1587005BBa_K1587005 Version 1 (Component)Butyrate synthesis pathway (BBa_1587004) without ccr gene regulated by constitutive promoter p(Bla)
BBa_K1523008BBa_K1523008 Version 1 (Component)A device can reduce Cr(VI)
BBa_K325109BBa_K325109 Version 1 (Component)EPIC Firefly Luciferase and LRE (under pBAD)<BR><i>P. Pyralis<BR>(E. coli optimised)</i>
CR-1C3-LacBBa_K590050 Version 1 (Component)ADC-AAR-PSB1C3-Lac Inducible
CR-1C3-HCBBa_K590049 Version 1 (Component)ADC-AAR-PSB1C3-High constitutive
BBa_J119405BBa_J119405 Version 1 (Component)(pSB1A2-BR) Deletion of the original -35 sequence
BBa_J119310BBa_J119310 Version 1 (Component)pSB1A2-BR-HS-G-IT-GGTT-RNA-I
BBa_K1058008BBa_K1058008 Version 1 (Component)Cr(VI) sensor with egfp(PchrB+chrB+egfp)
BBa_K1763003BBa_K1763003 Version 1 (Component)Major Spidroin Protein 2 (MaSp2) with sticky ends BC
BBa_K1114100BBa_K1114100 Version 1 (Component)MoClo format of a modified version of BBa_B0030 with BC fusion sites.
BBa_K1114102BBa_K1114102 Version 1 (Component)MoClo format of a modified version of BBa_B0032 with BC fusion sites
BBa_K1114101BBa_K1114101 Version 1 (Component)MoClo format of a modified version of BBa_B0031 with BC fusion sites.
BBa_K382033BBa_K382033 Version 1 (Component)EcR (Ecdysone Receptor)
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_J119309BBa_J119309 Version 1 (Component)pSB1A2-BR-HS-G-RNA-I
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.