ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
scarBBa_K112999 Version 1 (Component)BBb1 scar sequence
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_K1321340BBa_K1321340 Version 1 (Component)Double CBD (dCBD) with N-terminal linker
BBa_M36670BBa_M36670 Version 1 (Component)Bxb1 Recombinase + NLS on N terminus
NLSBBa_J176008 Version 1 (Component)NLS
rhIIBBa_C0070 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
BBa_K249008BBa_K249008 Version 1 (Component)YFP with N-terminal Arganine Tag
LacIBBa_K143033 Version 1 (Component)LacI (Lva<sup>-</sup>, N-terminal deletion) regulatory protein
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_K1362057BBa_K1362057 Version 1 (Component)NLS as RFC[105] N-terminal insert
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
BBa_K1423000BBa_K1423000 Version 1 (Component)BCLA N-terminal Domain
G-His6(N)BBa_K1362430 Version 1 (Component)C-terminal His6 (N-Intein)
NLS+TermBBa_K165018 Version 1 (Component)SV40 nuclear localization sequence + ADH1 transcription terminator
RFC105 NNBBa_K1362415 Version 1 (Component)N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
Bxb1_IntBBa_K907000 Version 1 (Component)Mycobacterium Phage Bxb1 gp35, DNA integrase
lacZ aBBa_E0033 Version 1 (Component)LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
BBa_K1362059BBa_K1362059 Version 1 (Component)PKI-NES as RFC[105] N-terminal insert
His-ScaffoBBa_K1602027 Version 1 (Component)Protein scaffold with N-terminal HisTag
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K331001BBa_K331001 Version 1 (Component)ECFP with N-terminal Arginine Tag
His6BBa_K1362431 Version 1 (Component)N-terminal His6 (C-Intein)
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
CBDcipABBa_K1615111 Version 1 (Component)CBDcipA with N and C-terminal linker in RFC25
BBa_K1491022BBa_K1491022 Version 1 (Component)N-terminus of T7 RNA Polymerase
BBa_K1321014BBa_K1321014 Version 1 (Component)CBDCipA with N and C-terminal linker
NpuDnaE(N)BBa_K1362400 Version 1 (Component)NpuDnaE N-Intein cloning piece
BBa_K1333005BBa_K1333005 Version 1 (Component)M13 bacteriophage g8p with a FLAG tag on C-terminal
BBa_K772002BBa_K772002 Version 1 (Component)EpCAM Binding Dock: N terminus
BBa_K1092105BBa_K1092105 Version 1 (Component)RFP N terminus
BBa_K1159109BBa_K1159109 Version 1 (Component)Micrococcal nuclease (Thermonuclease) with N-terminal SV40 NLS in RFC[25]
BBa_I712022BBa_I712022 Version 1 (Component)T7 RNA polymerase with NLS
BBa_K1446002BBa_K1446002 Version 1 (Component)superfolder GFP with N-terminal pdu-microcompartment tag
BBa_K364165BBa_K364165 Version 1 (Component)Zinc Finger Protein fixed N-terminus
BBa_I712069BBa_I712069 Version 1 (Component)RBS-T7 RNA polymerase with NLS
BBa_K2116026BBa_K2116026 Version 1 (Component)bxb1 integrase