BBa_K535007
BBa_K535007 Version 1 (Component)
pBBR1MCS-5 -> broad-host-range cloning vector

BBa_K615001
BBa_K615001 Version 1 (Component)
Zinc finger-omega subunit fusion protein

BBa_K654096
BBa_K654096 Version 1 (Component)
E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)

BBa_K896003
BBa_K896003 Version 1 (Component)
Sulfide quinone reductase(SQR) on neutral site 2 vector

BBa_K950010
BBa_K950010 Version 1 (Component)
pRS315 shuttle vector

BBa_K950011
BBa_K950011 Version 1 (Component)
pRS316 shuttle vector

BBa_J72202
BBa_J72202 Version 1 (Component)
ampicillin resistant p15A vector

BBa_J72203
BBa_J72203 Version 1 (Component)
kanamycin resistant split R6K vector

BBa_J72204
BBa_J72204 Version 1 (Component)
ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT

BBa_J72205
BBa_J72205 Version 1 (Component)
ampicillin resistant split ColE2 vector

BBa_J72206
BBa_J72206 Version 1 (Component)
ampicillin resistant split R6K vector

BBa_J72207
BBa_J72207 Version 1 (Component)
ampicillin resistant pUC vector

BBa_J72209
BBa_J72209 Version 1 (Component)
kanamycin + ampicillin resistant p15A vector for 2ab assembly

BBa_J72211
BBa_J72211 Version 1 (Component)
ampicillin resistant p15A vector with dapD

BBa_J72212
BBa_J72212 Version 1 (Component)
spectinomycin resistant pUC vector

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737021
BBa_K737021 Version 1 (Component)
gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

BBa_J72218
BBa_J72218 Version 1 (Component)
kanamycin resistant p15A vector

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Eukaryotic
BBa_K157040 Version 1 (Component)
Eukaryotic terminator vector (very similar to pSB1AK8)

Transfecti
BBa_K157041 Version 1 (Component)
Transfection vector (similar to pSB1AK8)

BBa_K106342
BBa_K106342 Version 1 (Component)
AB shuttle vector

BBa_K106343
BBa_K106343 Version 1 (Component)
BD shuttle vector

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K146000
BBa_K146000 Version 1 (Component)
pCR2.1 PCR product cloning vector

BBa_K106672
BBa_K106672 Version 1 (Component)
AarI AD acceptor vector (pRS305, Gal1P, Adh1t)

BBa_K105000
BBa_K105000 Version 1 (Component)
pSB1A2_fusion

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_K106697
BBa_K106697 Version 1 (Component)
AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)

BBa_K106698
BBa_K106698 Version 1 (Component)
AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

BBa_K106701
BBa_K106701 Version 1 (Component)
pCR2.1 TOPO Vector (to contain AarI inserts)

BBa_K165061
BBa_K165061 Version 1 (Component)
pRS304* yeast shuttle vector, TRP1 selection

BBa_K090402
BBa_K090402 Version 1 (Component)
B. subtilis Episomal Vector with Constitutive GFP

BBa_K165060
BBa_K165060 Version 1 (Component)
pRS303 yeast shuttle vector, HIS3

BBa_K165062
BBa_K165062 Version 1 (Component)
pRS305 yeast shuttle vector, LEU2 selection

BBa_K165063
BBa_K165063 Version 1 (Component)
pRS306 yeast shuttle vector, URA3 selection

BBa_K090403
BBa_K090403 Version 1 (Component)
Gram-positive Shuttle Vector for Chromosomal Integration

BBa_K112134
BBa_K112134 Version 1 (Component)
Cam-Amp assembly vector

BBa_K112143
BBa_K112143 Version 1 (Component)
Entry vector with OriV replication origin

AD default
BBa_K106400 Version 1 (Component)
Default insert for AarI AD acceptor vectors

BBa_J70034
BBa_J70034 Version 1 (Component)
A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)

BBa_J70037
BBa_J70037 Version 1 (Component)
Library Vector Test Part

BBa_J70040
BBa_J70040 Version 1 (Component)
A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)