BBa_K737009
BBa_K737009 Version 1 (Component)
GVPC and GFP coding

BBa_K737021
BBa_K737021 Version 1 (Component)
gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K873000
BBa_K873000 Version 1 (Component)
heat shock 10kDa protein(hsp 10) groES coding resquence

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_K883160
BBa_K883160 Version 1 (Component)
CCMV_Negative coat protein

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

mtrB
BBa_K098989 Version 1 (Component)
mtrB coding region

BBa_K098997
BBa_K098997 Version 1 (Component)
cIts coding region

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

BBa_I0466
BBa_I0466 Version 1 (Component)
RhlR Protein Generator

KaiA
BBa_J36801 Version 1 (Component)
KaiA coding region

KaiB
BBa_J36802 Version 1 (Component)
KaiB coding region

KaiC
BBa_J36804 Version 1 (Component)
KaiC coding region

BBa_J04430
BBa_J04430 Version 1 (Component)
GFP coding device switched on by IPTG

pLac+GFP
BBa_I763004 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K116601
BBa_K116601 Version 1 (Component)
HtlB (ftsH) coding region from E. coli

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K127011
BBa_K127011 Version 1 (Component)
cheR coding region

BBa_K127012
BBa_K127012 Version 1 (Component)
cheZ coding region

BBa_K127013
BBa_K127013 Version 1 (Component)
gas vesicle protein A from cyanobacteria Anabaena

BBa_J36835
BBa_J36835 Version 1 (Component)
Lipoprotein signal peptide

BBa_J36836
BBa_J36836 Version 1 (Component)
Outer membrane protein A, aa 46-66

BBa_K116102
BBa_K116102 Version 1 (Component)
IPTG inducible membrane protein generator

CII
BBa_K116602 Version 1 (Component)
CII coding region from λ phage

CIIICd
BBa_K116609 Version 1 (Component)
CIIICd: modified CIII coding region from λ phage