BBa_K581009
BBa_K581009 Version 1 (Component)
pqrr-GFP ssrA degradation tag

BBa_K581012
BBa_K581012 Version 1 (Component)
pqrr-rbs-T7ptag-terminator

BBa_K581013
BBa_K581013 Version 1 (Component)
lux repressible promoter-T7ptag-terminator

BBa_K581015
BBa_K581015 Version 1 (Component)
pBAD-supD-plux_inv-T7ptag

BBa_K654096
BBa_K654096 Version 1 (Component)
E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)

BBa_K588100
BBa_K588100 Version 1 (Component)
N-terminal leucine zipper

BBa_K823038
BBa_K823038 Version 1 (Component)
Strep-tag (Freiburg standard+RBS)

GFP-AID
BBa_K812010 Version 1 (Component)
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K863005
BBa_K863005 Version 1 (Component)
ecol laccase from E. coli with T7 promoter, RBS and His-tag

BBa_K863000
BBa_K863000 Version 1 (Component)
bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag

BBa_K863010
BBa_K863010 Version 1 (Component)
tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737021
BBa_K737021 Version 1 (Component)
gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

BM-CFP
BBa_K774004 Version 1 (Component)
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420

MB-CFP
BBa_K774006 Version 1 (Component)
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

BBa_K157012
BBa_K157012 Version 1 (Component)
Strep affinity tag II; Freiburg standard

lexA
BBa_K165020 Version 1 (Component)
LexA Synthetic Activator (untagged)

BBa_K165021
BBa_K165021 Version 1 (Component)
Kozak + LexA Synthetic Activator (untagged)

BBa_K165022
BBa_K165022 Version 1 (Component)
Zif268-HIV synthetic activator (untagged)

BBa_K165023
BBa_K165023 Version 1 (Component)
Kozak + Zif268-HIV synthetic activator (untagged)

BBa_K122014
BBa_K122014 Version 1 (Component)
Yeast ORI and His Tag

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K105000
BBa_K105000 Version 1 (Component)
pSB1A2_fusion

BBa_K112325
BBa_K112325 Version 1 (Component)
{< S-Tag!}{< b1006 >}

BBa_K112348
BBa_K112348 Version 1 (Component)
{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}

BBa_K112349
BBa_K112349 Version 1 (Component)
{rbs_pelB>} {< ligase>}{< S-Tag!}{< b1006>}

BBa_K112350
BBa_K112350 Version 1 (Component)
{rbs_phoApp>}{< r.BamHI>}{< S-Tag!}{< b1006>}

BBa_K112351
BBa_K112351 Version 1 (Component)
{rbs_lamB>} {< r.BglII>}{< S-Tag!}{< b1006>}

BBa_K112368
BBa_K112368 Version 1 (Component)
{Pbad}{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}

BBa_K112369
BBa_K112369 Version 1 (Component)
{Pbad}{rbs_pelB>} {< ligase>}{< S-Tag!}{< b1006>}

BBa_K112370
BBa_K112370 Version 1 (Component)
{Pbad}{rbs_phoApp>}{< r.BamHI>}{< S-Tag!}{< b1006>}

BBa_K112371
BBa_K112371 Version 1 (Component)
{Pbad}{rbs_lamB>} {< r.BglII>}{< S-Tag!}{< b1006>}

BBa_I13601
BBa_I13601 Version 1 (Component)
Lac operator with CFP reporter (without LVA tag) [R/Lc-]

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K165049
BBa_K165049 Version 1 (Component)
Full LexA Repressor (tagges with mCherryx2)

BBa_K165050
BBa_K165050 Version 1 (Component)
Full Gli1 Repressor (tagged with mCherryx2)

BBa_K165051
BBa_K165051 Version 1 (Component)
Full YY1 Repressor (tagged with mCherryx2)

BBa_K165052
BBa_K165052 Version 1 (Component)
Full Zif268-HIV Repressor (tagged with mCherryx2)

BBa_K165053
BBa_K165053 Version 1 (Component)
Full LexA Repressor (tagged with CFPx2)

BBa_K165054
BBa_K165054 Version 1 (Component)
Full Gli1 Repressor (tagged with CFPx2)