ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_J61101BBa_J61101 Version 1 (Component)Ribosome Binding Site Family Member
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_J61120BBa_J61120 Version 1 (Component)Ribosome Binding Site Family Member
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
BBa_J61100BBa_J61100 Version 1 (Component)Ribosome Binding Site Family Member
BBa_I712015BBa_I712015 Version 1 (Component)HIV-1 protease cleavage site
BBa_J85600BBa_J85600 Version 1 (Component)attB1 recombination site
EcoRIEcoRI Version 1 (Component) NheINheI Version 1 (Component) PacIPacI Version 1 (Component) SpeISpeI Version 1 (Component) BBa_I712004BBa_I712004 Version 1 (Component)CMV promoter
preSCsiteBBa_J18919 Version 1 (Component)preScission protease cleavage site (E. coli-optimized)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_I712043BBa_I712043 Version 1 (Component)CMVp - myristoilation site - HIV protease cleavage site - T7RNAP - NLS
BBa_I712044BBa_I712044 Version 1 (Component)CMVp - myristoilation site - HIV protease cleavage site - mCherry - NLS
BBa_I712034BBa_I712034 Version 1 (Component)CMVp - myristoilation site - HIV protease cleavage site
RBS_cassetteRBS_cassette Version 1 (Component)AvrII restriction site (cctagg), overlapped with RBS (aggagga)
BBa_I712045BBa_I712045 Version 1 (Component)CMVp - myristoilation site - HIV protease cleavage site - GFP
BBa_I712016BBa_I712016 Version 1 (Component)myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site
loxBBa_J61046 Version 1 (Component)[Lox] site for recombination
BBa_I712047BBa_I712047 Version 1 (Component)CMVp - HA - CD4 - HIV protease cleavage site - T7RNAP - NLS
BBa_I13457BBa_I13457 Version 1 (Component)Improved insertion site with two RNAse E cut sites
RBS-spoVGBBa_K143021 Version 1 (Component)SpoVG ribosome binding site (RBS) for B. subtilis
BBa_I13452BBa_I13452 Version 1 (Component)Adds another BioBrick site
NLSBBa_J176008 Version 1 (Component)NLS
BBa_K165002BBa_K165002 Version 1 (Component)Kozak sequence (yeast RBS)
PBS_F24PBS_F24 Version 1 (Component) BBa_K1123012BBa_K1123012 Version 1 (Component)His-Tag and Thrombine Cleavage Site
TEV clv.BBa_K1362453 Version 1 (Component)TEV protease recognition/cleavage site
BBa_I712041BBa_I712041 Version 1 (Component)CMVp - CD4 - HIV protease cleavage site - mCherryNLS
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS
TEVsiteBBa_J18918 Version 1 (Component)TEV cleavage site (E. coli)