g3p
BBa_K257001 Version 1 (Component)
Gene 3 protein of filamentous phages without the 18 first amino acids

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K109551
BBa_K109551 Version 1 (Component)
TrbA transcription factor for the Tra2 operon in incP alpha conjugation

BBa_K1319007
BBa_K1319007 Version 1 (Component)
6xHis tag with double stop codon

BBa_K2055019
BBa_K2055019 Version 1 (Component)
Part designed for verification and characterization of the heat the shock promoter and Inverter.

BBa_I12056
BBa_I12056 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12036

BBa_I12055
BBa_I12055 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12006

BBa_I12057
BBa_I12057 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12040

BBa_J72096
BBa_J72096 Version 1 (Component)
{araC-Pbad}{rbs.coi}{double terminator}{repL no start}{LPsit}{rbs.pacA}{TrnpB}

BBa_K1509002
BBa_K1509002 Version 1 (Component)
Mainly responsible for the flocculation of <em>Bacillus sp. F2</em>

BBa_K1051307
BBa_K1051307 Version 1 (Component)
k1051304(cln3 promoter)+k1051062(K1051004 (non stop codon morange) + K1051006 (Stop codon +TBY-1term

BBa_I13977
BBa_I13977 Version 1 (Component)
intermediate for lac relaxation oscillator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K163001
BBa_K163001 Version 1 (Component)
Mussel adhesive protein from Mytilus Edulis (Mefp-5) optimized for Caulobacter

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_K1114108
BBa_K1114108 Version 1 (Component)
Bicistronic design RBS 8, MoClo Format with BC fusion sites

BBa_K081019
BBa_K081019 Version 1 (Component)
luxR protein generator under Plambda regulation

BBa_K1096001
BBa_K1096001 Version 1 (Component)
MazE protein (E. coli)

BBa_K812032
BBa_K812032 Version 1 (Component)
mCFP with kozak sequence for eukaryotic expression

BBa_I13535
BBa_I13535 Version 1 (Component)
Test construct for screening plasmid

BBa_M30110
BBa_M30110 Version 1 (Component)
part of the M13 viral genome for annotation/refactoring

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

PgpdA
BBa_K678024 Version 1 (Component)
PgpdA, promoter for Aspergillus nidulans

BBa_J54015
BBa_J54015 Version 1 (Component)
Protein Binding Site_LacI

BBa_K1163400
BBa_K1163400 Version 1 (Component)
AceB promoter, iron sensitive, down-regulated by dimerized FUR protein

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

ompC w/Cad
BBa_M11084 Version 1 (Component)
E coli outer membrane protein C (ompC) with Cadmium Binding protien

BBa_K737049
BBa_K737049 Version 1 (Component)
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42

BBa_K737048
BBa_K737048 Version 1 (Component)
The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42

fhlA
BBa_K778001 Version 1 (Component)
fhlA: a transcriptional activator for enhanced hydrogen production

BBa_J52028
BBa_J52028 Version 1 (Component)
green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag

BBa_K2055007
BBa_K2055007 Version 1 (Component)
LacI for Acidithiobacillus

BBa_J07060
BBa_J07060 Version 1 (Component)
FecA::scFv internal fusion type 2 @ aa522-532

BBa_J07063
BBa_J07063 Version 1 (Component)
FecA::scFv internal fusion type 2 @ aa566-577

iGEM 2019 Distribution
iGEM2019Distribution_collection Version 1 (Collection)
Distribution of parts for the 2019 iGEM competition

iGEM 2018 Distribution
iGEM2018Distribution_collection Version 1 (Collection)
Distribution of parts for the 2018 iGEM competition

BBa_I20275
BBa_I20275 Version 1 (Component)
LacI protein generator

BBa_I20272
BBa_I20272 Version 1 (Component)
LacI protein generator

BBa_K953001
BBa_K953001 Version 1 (Component)
Deinococcus radiodurans Bacteriophytochrome with RBS (E. coli Codon Optimised)

BBa_K1424000
BBa_K1424000 Version 1 (Component)
Prefix homologous recombination for Synechocystis ("Left flank")

Tn5F
BBa_J61021 Version 1 (Component)
[Tn5-for]

BBa_J56006
BBa_J56006 Version 1 (Component)
Codon-optimized signaling domain, cytoplasmic domain of Tar

KWR
BBa_K175030 Version 1 (Component)
Key for lock of weak (K175029) RBS (B0031) from the lock/key library TUD09

BBa_R2109
BBa_R2109 Version 1 (Component)
Promoter with operator site for C2003

BBa_K1695030
BBa_K1695030 Version 1 (Component)
RBS + Bacteriophage &#955; codon-optimized S mutant gene

BBa_K1491021
BBa_K1491021 Version 1 (Component)
Constitutive Codon Optimized Super Nova

BBa_K1491020
BBa_K1491020 Version 1 (Component)
Constitutive Codon Optimized Killer Red

BBa_K1420003
BBa_K1420003 Version 1 (Component)
MerP, mercuric transport protein periplasmic component