BBa_J70404BBa_J70404 Version 1 (Component)R0010.E0021.J70394.E0031 or J70398.E0031
BBa_J70403BBa_J70403 Version 1 (Component)R0010.E0031.J70394.E0021 or J70397.E0021
BBa_K2123206BBa_K2123206 Version 1 (Component)Strong RBS + Organomercurial Lyase (MerB) for E. coli
BBa_M50016BBa_M50016 Version 1 (Component)Promoter, RBS and GolS Activator for Gold Detection
BBa_K648131BBa_K648131 Version 1 (Component)Pr of Or tester with cI repressor
BBa_K327017BBa_K327017 Version 1 (Component)lux OR delta cI activated "off" part
BBa_K812132BBa_K812132 Version 1 (Component)mCFP with kozak sequence for expression in Xenopus
BBa_K541000BBa_K541000 Version 1 (Component)Limulus anti-LPS factor (LALF) for Bacillus subtilis
BBa_K812133BBa_K812133 Version 1 (Component)sfGFP with kozak sequence for expression in Xenopus
BBa_K077025BBa_K077025 Version 1 (Component)HSL/LuxR or LasR promotor test system
BBa_J70368BBa_J70368 Version 1 (Component)I13502 or K093005 (B0034+E1010) rfp without PsrI sites
Holin, T4BBa_K112003 Version 1 (Component)T4 holin, without start or stop codon, berkeley standard
BBa_J329004BBa_J329004 Version 1 (Component)Quorum Sensing Sender Part for Use in BBa_J329003
BBa_K415004BBa_K415004 Version 1 (Component)P_const(high) : RBS : LuxR : Term : pLux/CI-OR
BBa_K1132038BBa_K1132038 Version 1 (Component)XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter
BBa_M31748BBa_M31748 Version 1 (Component)Gene VII w/o Genes IX RBS or VIII Promoter
BBa_J70552BBa_J70552 Version 1 (Component)J70551.J70549 (or J70548.J70550.J70549) composite part
BBa_K750108BBa_K750108 Version 1 (Component)PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose
BBa_K2123301BBa_K2123301 Version 1 (Component)Novel Synthetic Phytochelatin codon optimized for E. coli expression
KanR-BA-BfBBa_K349012 Version 1 (Component)KanR-BA-BfuAI (for construction of BA BioBytes 2.0)
BBa_M31002BBa_M31002 Version 1 (Component)Refactored Gene 2 without Gene 10's Promoter or RBS
BBa_K1363611BBa_K1363611 Version 1 (Component)key-1 of gate-of-or of RNA logic gates
BBa_K415003BBa_K415003 Version 1 (Component)P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR
BBa_K112222BBa_K112222 Version 1 (Component){< ihfB >} The ihf beta gene without start or stop codons, BBb format
BBa_K563010BBa_K563010 Version 1 (Component)Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway
PchiP-lacZBBa_K564002 Version 1 (Component)Chitoporin fused with lacZ - target for sRNA based regulation
BBa_J70556BBa_J70556 Version 1 (Component)BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K812130BBa_K812130 Version 1 (Component)Citrine reporter with a Kozak sequence for expression in Xenopus
BBa_K812120BBa_K812120 Version 1 (Component)IaaH on PSC2+ Ready for use in xenopus tropicalis egg
BBa_K09100BBa_K09100 Version 1 (Component)Receiver for AHL and Outputs GFP when AHL is present
BBa_K648125BBa_K648125 Version 1 (Component)Or tester with cI repressor with slightly weak RBS
BBa_K648126BBa_K648126 Version 1 (Component)Or tester with cI repressor with very weak RBS
BBa_K251000BBa_K251000 Version 1 (Component)coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)
BBa_I714001BBa_I714001 Version 1 (Component)trwC of R388 ( relaxase or nickase of the R388 conjugative plasmid )
BBa_K415002BBa_K415002 Version 1 (Component)P_const(high) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term
xis domainBBa_K112203 Version 1 (Component){< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21
BBa_J70559BBa_J70559 Version 1 (Component)N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040
BBa_K363002BBa_K363002 Version 1 (Component)A calcium dependent response element binding site for the Crz1 activator
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K802003BBa_K802003 Version 1 (Component)Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>
BBa_K1942000BBa_K1942000 Version 1 (Component)A shRNA corresponding DNA sequence for KRAS which could silence the gene
BBa_K2016006BBa_K2016006 Version 1 (Component)Superfolder GFP under control or medium constitutive promoter and RyhB siRNA domain
BBa_K415001BBa_K415001 Version 1 (Component)P_const(low/med) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term
BBa_K750115BBa_K750115 Version 1 (Component)pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)
BBa_K726009BBa_K726009 Version 1 (Component)T7 driven lac operated inducer for the rhl quorum-sensing system